Proteomic analysis of the cardiac myocyte secretome reveals extracellular protective functions for the ER stress response

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F20%3A00524331" target="_blank" >RIV/68081715:_____/20:00524331 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0308692" target="_blank" >http://hdl.handle.net/11104/0308692</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.yjmcc.2020.04.012" target="_blank" >10.1016/j.yjmcc.2020.04.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteomic analysis of the cardiac myocyte secretome reveals extracellular protective functions for the ER stress response

Popis výsledku v původním jazyce

The effect of ER stress on protein secretion by cardiac myocytes using ER stressors thapsigargin (TG), which depletes ER calcium, and tunicamycin (TM) was studied. Proteomic analyses of TG- and TM-conditioned media showed that the secretion of most proteins was inhibited by TG and TM, however, secretion of several ER-resident proteins, including GRP78 was increased by TG but not TM. Simulated ischemia, which decreases SR/ER calcium also increased secretion of these proteins. The proteins secreted during ER stress mediated by ER calcium depletion can enhance cardiac myocyte viability.

Název v anglickém jazyce

Proteomic analysis of the cardiac myocyte secretome reveals extracellular protective functions for the ER stress response

Popis výsledku anglicky

The effect of ER stress on protein secretion by cardiac myocytes using ER stressors thapsigargin (TG), which depletes ER calcium, and tunicamycin (TM) was studied. Proteomic analyses of TG- and TM-conditioned media showed that the secretion of most proteins was inhibited by TG and TM, however, secretion of several ER-resident proteins, including GRP78 was increased by TG but not TM. Simulated ischemia, which decreases SR/ER calcium also increased secretion of these proteins. The proteins secreted during ER stress mediated by ER calcium depletion can enhance cardiac myocyte viability.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Molecular and Cellular Cardiology

ISSN

0022-2828

e-ISSN

—

Svazek periodika

143

Číslo periodika v rámci svazku

JUN

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

132-144

Kód UT WoS článku

000579927300012

EID výsledku v databázi Scopus

2-s2.0-85084362964