Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F24%3A00584554" target="_blank" >RIV/68081715:_____/24:00584554 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/24:00135448

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0021967324000426?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967324000426?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2024.464669" target="_blank" >10.1016/j.chroma.2024.464669</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

Popis výsledku v původním jazyce

Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LCMS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LCMS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LCMS analysis of human insulin.

Název v anglickém jazyce

Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

Popis výsledku anglicky

Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LCMS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LCMS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LCMS analysis of human insulin.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

1873-3778

Svazek periodika

1717

Číslo periodika v rámci svazku

FEB

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

464669

Kód UT WoS článku

001173156700001

EID výsledku v databázi Scopus

2-s2.0-85185009025