Investigation of interactions between biological thiols and gold nanoparticles by capillary electrophoresis with laser-induced fluorescence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F24%3A00599201" target="_blank" >RIV/68081715:_____/24:00599201 - isvavai.cz</a>

Výsledek na webu

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202300248" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202300248</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.202300248" target="_blank" >10.1002/elps.202300248</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Investigation of interactions between biological thiols and gold nanoparticles by capillary electrophoresis with laser-induced fluorescence

Popis výsledku v původním jazyce

Biological thiols spontaneously form a stable Au-S dative bond with gold nanoparticles (AuNP) that might be used for their selective extraction and enrichment in biological samples. In this work, interactions of selected biological thiols (glutathione, cysteine, homocysteine [Hcys], cysteamine [CA], and N-acetylcysteine) with AuNP stabilized by different capping agents (citrate, Tween 20, Brij 35, CTAB, SDS) were investigated by UV-Vis spectroscopy and capillary electrophoresis with laser-induced fluorescence. Spectrophotometric measurements showed aggregation of Hcys and CA with AuNP. In contrast, it was confirmed by CE-LIF that biological thiols were adsorbed to all types of AuNP. Citrate-capped AuNP were selected for AuNP-based extraction of biological thiols from exhaled breath condensate (EBC). Dithiothreitol was utilized for desorption of biological thiols from the AuNP surface, which was followed by derivatization with eosin-5-maleimide and CE-LIF analysis. AuNP-based extraction increased the sensitivity of CE-LIF analysis, however, further optimization of methodology is necessary for accurate quantification of biological thiols in EBC.

Název v anglickém jazyce

Investigation of interactions between biological thiols and gold nanoparticles by capillary electrophoresis with laser-induced fluorescence

Popis výsledku anglicky

Biological thiols spontaneously form a stable Au-S dative bond with gold nanoparticles (AuNP) that might be used for their selective extraction and enrichment in biological samples. In this work, interactions of selected biological thiols (glutathione, cysteine, homocysteine [Hcys], cysteamine [CA], and N-acetylcysteine) with AuNP stabilized by different capping agents (citrate, Tween 20, Brij 35, CTAB, SDS) were investigated by UV-Vis spectroscopy and capillary electrophoresis with laser-induced fluorescence. Spectrophotometric measurements showed aggregation of Hcys and CA with AuNP. In contrast, it was confirmed by CE-LIF that biological thiols were adsorbed to all types of AuNP. Citrate-capped AuNP were selected for AuNP-based extraction of biological thiols from exhaled breath condensate (EBC). Dithiothreitol was utilized for desorption of biological thiols from the AuNP surface, which was followed by derivatization with eosin-5-maleimide and CE-LIF analysis. AuNP-based extraction increased the sensitivity of CE-LIF analysis, however, further optimization of methodology is necessary for accurate quantification of biological thiols in EBC.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA22-23815S" target="_blank" >GA22-23815S: Využití kovových nanočástic pro selektivní extrakci biologických thiolů v neinvazivních vzorcích.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

1522-2683

Svazek periodika

45

Číslo periodika v rámci svazku

15-16

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1418-1427

Kód UT WoS článku

001176919600001

EID výsledku v databázi Scopus

2-s2.0-85181654053