A Method for Determination of Transport Efficiency in Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Tissue Analysis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00636911" target="_blank" >RIV/68081715:_____/25:00636911 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14310/25:00141959
Výsledek na webu
<a href="https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.5c01306?ref=article_openPDF" target="_blank" >https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.5c01306?ref=article_openPDF</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.5c01306" target="_blank" >10.1021/acs.analchem.5c01306</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
A Method for Determination of Transport Efficiency in Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Tissue Analysis
Popis výsledku v původním jazyce
Recent studies have demonstrated the applicability of the single particle mode in laser ablation-inductively coupled plasma-mass spectrometry to map and size particles simultaneously. The transport efficiency (TE) is an important parameter in this configuration and affects the detection of individual nanoparticles, reliability of nanoparticle characterization, and related applications. This study introduces a novel method for the precise determination of TE, based on counting upconversion nanoparticles from gels characterized by fluorescent microscopy. The method was found to be most suitable for the 2940-nm laser ablation system, achieving virtually quantitative nanoparticle desorption, with TE primarily governed by ablation cell design and aerosol transport efficiency. With the 213-nm laser, attention had to be paid to incomplete desorption and possible nanoparticle redeposition at low laser fluences to avoid variability in TE measurements. Finally, use of the 193-nm laser-induced nanoparticle disintegration, resulting in elevated baseline noise and lower sensitivity, which prevented the use of this approach for the determination of TE. This study highlights the versatility of the proposed method, while also identifying its limitations, in terms of wavelength and fluence.
Název v anglickém jazyce
A Method for Determination of Transport Efficiency in Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Tissue Analysis
Popis výsledku anglicky
Recent studies have demonstrated the applicability of the single particle mode in laser ablation-inductively coupled plasma-mass spectrometry to map and size particles simultaneously. The transport efficiency (TE) is an important parameter in this configuration and affects the detection of individual nanoparticles, reliability of nanoparticle characterization, and related applications. This study introduces a novel method for the precise determination of TE, based on counting upconversion nanoparticles from gels characterized by fluorescent microscopy. The method was found to be most suitable for the 2940-nm laser ablation system, achieving virtually quantitative nanoparticle desorption, with TE primarily governed by ablation cell design and aerosol transport efficiency. With the 213-nm laser, attention had to be paid to incomplete desorption and possible nanoparticle redeposition at low laser fluences to avoid variability in TE measurements. Finally, use of the 193-nm laser-induced nanoparticle disintegration, resulting in elevated baseline noise and lower sensitivity, which prevented the use of this approach for the determination of TE. This study highlights the versatility of the proposed method, while also identifying its limitations, in terms of wavelength and fluence.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Analytical Chemistry
ISSN
0003-2700
e-ISSN
1520-6882
Svazek periodika
97
Číslo periodika v rámci svazku
25
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
12940-12946
Kód UT WoS článku
001510151300001
EID výsledku v databázi Scopus
2-s2.0-105008272918