Sieve element occlusion (SEO) genes encode structural phloem proteins involved in wound sealing of the phloem
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F12%3A00385330" target="_blank" >RIV/68081731:_____/12:00385330 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1073/pnas.1202999109" target="_blank" >http://dx.doi.org/10.1073/pnas.1202999109</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.1202999109" target="_blank" >10.1073/pnas.1202999109</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Sieve element occlusion (SEO) genes encode structural phloem proteins involved in wound sealing of the phloem
Popis výsledku v původním jazyce
The sieve element occlusion (SEO) gene family originally was delimited to genes encoding structural components of forisomes, which are specialized crystalloid phloem proteins found solely in the Fabaceae. More recently, SEO genes discovered in various non-Fabaceae plants were proposed to encode the common phloem proteins (P-proteins) that plug sieve plates after wounding. We carried out a comprehensive characterization of two tobacco (Nicotiana tabacum) SEO genes (NtSEO). Reporter genes controlled by the NtSEO promoters were expressed specifically in immature sieve elements, and GFP-SEO fusion proteins formed parietal agglomerates in intact sieve elements as well as sieve plate plugs after wounding. NtSEO proteins with and without fluorescent protein tags formed agglomerates similar in structure to native P-protein bodies when transiently coexpressed in Nicotiana benthamiana, and the analysis of these protein complexes by electron microscopy revealed ultrastructural features resembling
Název v anglickém jazyce
Sieve element occlusion (SEO) genes encode structural phloem proteins involved in wound sealing of the phloem
Popis výsledku anglicky
The sieve element occlusion (SEO) gene family originally was delimited to genes encoding structural components of forisomes, which are specialized crystalloid phloem proteins found solely in the Fabaceae. More recently, SEO genes discovered in various non-Fabaceae plants were proposed to encode the common phloem proteins (P-proteins) that plug sieve plates after wounding. We carried out a comprehensive characterization of two tobacco (Nicotiana tabacum) SEO genes (NtSEO). Reporter genes controlled by the NtSEO promoters were expressed specifically in immature sieve elements, and GFP-SEO fusion proteins formed parietal agglomerates in intact sieve elements as well as sieve plate plugs after wounding. NtSEO proteins with and without fluorescent protein tags formed agglomerates similar in structure to native P-protein bodies when transiently coexpressed in Nicotiana benthamiana, and the analysis of these protein complexes by electron microscopy revealed ultrastructural features resembling
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
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Svazek periodika
109
Číslo periodika v rámci svazku
28
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
"E1980"-"E1989"
Kód UT WoS článku
000306642100014
EID výsledku v databázi Scopus
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