Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F18%3A00498740" target="_blank" >RIV/68081731:_____/18:00498740 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1117/12.2517933" target="_blank" >http://dx.doi.org/10.1117/12.2517933</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2517933" target="_blank" >10.1117/12.2517933</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips

Popis výsledku v původním jazyce

Analyzing the cells in various body fluids can greatly deepen the understanding of the mechanisms governing the cellular physiology. Because of the variability of physiological and metabolic states, it is important to be able to perform such studies on individual cells. Therefore, we developed an optofluidic system in which we precisely manipulated and monitored individual cells of Escherichia coli. We used laser tweezers Raman spectroscopy (LTRS) in a microchamber chip to manipulate and analyze individual E. coli cells. We subjected the cells to antibiotic cefotaxime, and we observed the changes by the time-lapse microscopy and Raman spectroscopy. We found observable changes in the cellularnmorphology (cell elongation) and in Raman spectra, which were consistent with other recently published observations. We tested the capabilities of the optofluidic system and found it to be a reliable and versatile solution for this class of microbiological experiments.

Název v anglickém jazyce

Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips

Popis výsledku anglicky

Analyzing the cells in various body fluids can greatly deepen the understanding of the mechanisms governing the cellular physiology. Because of the variability of physiological and metabolic states, it is important to be able to perform such studies on individual cells. Therefore, we developed an optofluidic system in which we precisely manipulated and monitored individual cells of Escherichia coli. We used laser tweezers Raman spectroscopy (LTRS) in a microchamber chip to manipulate and analyze individual E. coli cells. We subjected the cells to antibiotic cefotaxime, and we observed the changes by the time-lapse microscopy and Raman spectroscopy. We found observable changes in the cellularnmorphology (cell elongation) and in Raman spectra, which were consistent with other recently published observations. We tested the capabilities of the optofluidic system and found it to be a reliable and versatile solution for this class of microbiological experiments.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

21st Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics. (Proceedings of SPIE 10976)

ISBN

9781510626072

ISSN

0277-786X

e-ISSN

—

Počet stran výsledku

8

Strana od-do

—

Název nakladatele

SPIE

Místo vydání

Bellingham

Místo konání akce

Lednice

Datum konání akce

3. 9. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000455324600043