Gold nanosystems for the detection of molecules using surface-enhanced Raman scatterings (SERS)

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Gold nanosystems for the detection of molecules using surface-enhanced Raman scatterings (SERS)

Popis výsledku v původním jazyce

Raman spectroscopy is a non-destructive analytical technique to analyze the chemical structure of molecules by a phenomenon known as Raman scattering, which occurs by an inelastic interaction of photons with the valence electrons in molecular bonds. However, Raman scattering can be hard to observe due to other, more frequent phenomena, such as Rayleigh scattering or fluorescence. SERS (surface-enhanced Raman spectroscopy) uses localized surface plasmon resonance (LSPR) of metal nanostructures to amplify Raman scattering. LSPR is a coherent oscillation of conduction electrons that arises from the interaction of electromagnetic radiation with metal nanostructures. The amplification of Raman scattering occurs when the analyte is adsorbed on the surface of such nanostructure and the strong localized electric field interacts with the electrons in its molecular bonds. Signal amplification of several orders of magnitude can be achieved, commonly 103 or more. In our work, we determined the presence of a selected bacterial species by multi-functionalized golden nanoparticles called SERS-tags, which have their surface modified with an antibody and a Raman reporter. The antibody allows the nanoparticles to bind to the surface of a concrete bacterial species based on the antigen-antibody affinity. When the targeted bacterium is covered with the nanoparticles, the Raman reporter signal is amplified by SERS, providing specific and strong Raman response. Therefore, when the Raman reporter signal is detected in a sample, it confirms the presence of the specific bacterium on a single-cell level.

Název v anglickém jazyce

Gold nanosystems for the detection of molecules using surface-enhanced Raman scatterings (SERS)

Popis výsledku anglicky

Raman spectroscopy is a non-destructive analytical technique to analyze the chemical structure of molecules by a phenomenon known as Raman scattering, which occurs by an inelastic interaction of photons with the valence electrons in molecular bonds. However, Raman scattering can be hard to observe due to other, more frequent phenomena, such as Rayleigh scattering or fluorescence. SERS (surface-enhanced Raman spectroscopy) uses localized surface plasmon resonance (LSPR) of metal nanostructures to amplify Raman scattering. LSPR is a coherent oscillation of conduction electrons that arises from the interaction of electromagnetic radiation with metal nanostructures. The amplification of Raman scattering occurs when the analyte is adsorbed on the surface of such nanostructure and the strong localized electric field interacts with the electrons in its molecular bonds. Signal amplification of several orders of magnitude can be achieved, commonly 103 or more. In our work, we determined the presence of a selected bacterial species by multi-functionalized golden nanoparticles called SERS-tags, which have their surface modified with an antibody and a Raman reporter. The antibody allows the nanoparticles to bind to the surface of a concrete bacterial species based on the antigen-antibody affinity. When the targeted bacterium is covered with the nanoparticles, the Raman reporter signal is amplified by SERS, providing specific and strong Raman response. Therefore, when the Raman reporter signal is detected in a sample, it confirms the presence of the specific bacterium on a single-cell level.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

LA62. Sborník příspěvků multioborové konference LASER62

ISBN

978-80-87441-30-5

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

9-11

Název nakladatele

Ústav přístrojové techniky AV ČR

Místo vydání

Brno

Místo konání akce

Lednice

Datum konání akce

9. 11. 2022

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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