Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F24%3A00587565" target="_blank" >RIV/68081731:_____/24:00587565 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26220/24:PU151557 RIV/00216224:14110/24:00136796 RIV/65269705:_____/24:00080246

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2001037024002204" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2001037024002204</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.csbj.2024.06.023" target="_blank" >10.1016/j.csbj.2024.06.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum

Popis výsledku v původním jazyce

Purple photosynthetic bacteria (PPB) are versatile microorganisms capable of producing various value-added chemicals, e.g., biopolymers and biofuels. They employ diverse metabolic pathways, allowing them to adapt to various growth conditions and even extreme environments. Thus, they are ideal organisms for the Next Generation Industrial Biotechnology concept of reducing the risk of contamination by using naturally robust extremophiles. Unfortunately, the potential of PPB for use in biotechnology is hampered by missing knowledge on regulations of their metabolism. Although Rhodospirillum rubrum represents a model purple bacterium studied for polyhydroxyalkanoate and hydrogen production, light/chemical energy conversion, and nitrogen fixation, little is known regarding the regulation of its metabolism at the transcriptomic level. Using RNA sequencing, we compared gene expression during the cultivation utilizing fructose and acetate as substrates in case of the wild-type strain R. rubrum DSM 467T and its knock-out mutant strain that is missing two polyhydroxyalkanoate synthases PhaC1 and PhaC2. During this first genome-wide expression study of R. rubrum, we were able to characterize cultivation-driven transcriptomic changes and to annotate non-coding elements as small RNAs. (c) 2024 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open accessnarticle under the CC BY-NC-ND license.

Název v anglickém jazyce

Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum

Popis výsledku anglicky

Purple photosynthetic bacteria (PPB) are versatile microorganisms capable of producing various value-added chemicals, e.g., biopolymers and biofuels. They employ diverse metabolic pathways, allowing them to adapt to various growth conditions and even extreme environments. Thus, they are ideal organisms for the Next Generation Industrial Biotechnology concept of reducing the risk of contamination by using naturally robust extremophiles. Unfortunately, the potential of PPB for use in biotechnology is hampered by missing knowledge on regulations of their metabolism. Although Rhodospirillum rubrum represents a model purple bacterium studied for polyhydroxyalkanoate and hydrogen production, light/chemical energy conversion, and nitrogen fixation, little is known regarding the regulation of its metabolism at the transcriptomic level. Using RNA sequencing, we compared gene expression during the cultivation utilizing fructose and acetate as substrates in case of the wild-type strain R. rubrum DSM 467T and its knock-out mutant strain that is missing two polyhydroxyalkanoate synthases PhaC1 and PhaC2. During this first genome-wide expression study of R. rubrum, we were able to characterize cultivation-driven transcriptomic changes and to annotate non-coding elements as small RNAs. (c) 2024 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open accessnarticle under the CC BY-NC-ND license.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20201 - Electrical and electronic engineering

Projekt

<a href="/cs/project/GF21-15958L" target="_blank" >GF21-15958L: Biologická funkce a dynamika PHA cyklu u bakterie Rhodospirillum rubrum a jeho biotechnologické konsekvence</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Computational and Structural Biotechnology Journal

ISSN

2001-0370

e-ISSN

2001-0370

Svazek periodika

23

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

2681-2694

Kód UT WoS článku

001261996700001

EID výsledku v databázi Scopus

2-s2.0-85196845882