Multiscale analysis of mechanical and structural properties of agarose-silk fibroin hydrogels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F25%3A00640852" target="_blank" >RIV/68081731:_____/25:00640852 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21220/25:00385679 RIV/00216305:26310/26:0199743

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0141813025086908" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0141813025086908</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2025.148133" target="_blank" >10.1016/j.ijbiomac.2025.148133</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiscale analysis of mechanical and structural properties of agarose-silk fibroin hydrogels

Popis výsledku v původním jazyce

This study provides a comprehensive characterization of the agarose-silk fibroin hydrogels, using atomic force microscopy and scanning transmission electron microscopy to analyse their structure and assess the effect of composition on mechanical properties via nanoindentation and rheological analysis. These measurements enabled determination of mechanical properties, including the elastic and viscoelastic moduli at both the microand macroscale. The hydrogels exhibited a wide range of moduli depending on different degrees of network crosslinking, influenced by varying concentrations of agarose (1 or 2 wt%) and the percentage of fibroin fibres (0-4.5 wt%) as an interpenetrating component. The viscoelastic modulus (G') and the elastic modulus determined using a relaxation model (E), were 5-57 kPa and 1.2-110 kPa, respectively. The adhesion energy of these hydrogels was determined from nanoindentation curves and analysed using the JKR model, with values ranging from 0.031 to 0.066 J m-2. These results provide insight into how the hydrogels' microstructure influences their mechanical and transport properties. Incorporating fibroin into these gels modifies biological and biochemical characteristics of the gels, suggesting that such composite hydrogels could be further explored for potential applications in controlled release systems, extracellular matrix models, or tissue engineering scaffolds.

Název v anglickém jazyce

Multiscale analysis of mechanical and structural properties of agarose-silk fibroin hydrogels

Popis výsledku anglicky

This study provides a comprehensive characterization of the agarose-silk fibroin hydrogels, using atomic force microscopy and scanning transmission electron microscopy to analyse their structure and assess the effect of composition on mechanical properties via nanoindentation and rheological analysis. These measurements enabled determination of mechanical properties, including the elastic and viscoelastic moduli at both the microand macroscale. The hydrogels exhibited a wide range of moduli depending on different degrees of network crosslinking, influenced by varying concentrations of agarose (1 or 2 wt%) and the percentage of fibroin fibres (0-4.5 wt%) as an interpenetrating component. The viscoelastic modulus (G') and the elastic modulus determined using a relaxation model (E), were 5-57 kPa and 1.2-110 kPa, respectively. The adhesion energy of these hydrogels was determined from nanoindentation curves and analysed using the JKR model, with values ranging from 0.031 to 0.066 J m-2. These results provide insight into how the hydrogels' microstructure influences their mechanical and transport properties. Incorporating fibroin into these gels modifies biological and biochemical characteristics of the gels, suggesting that such composite hydrogels could be further explored for potential applications in controlled release systems, extracellular matrix models, or tissue engineering scaffolds.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

1879-0003

Svazek periodika

330

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

148133

Kód UT WoS článku

001596413500014

EID výsledku v databázi Scopus

2-s2.0-105018055217