The characterization of halophilic polyhydroxyalkanoate-producing bacteria from brine in Solivar near Prešov (Slovakia)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F25%3A00643166" target="_blank" >RIV/68081731:_____/25:00643166 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26310/26:0200061

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11274-025-04737-5" target="_blank" >https://link.springer.com/article/10.1007/s11274-025-04737-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11274-025-04737-5" target="_blank" >10.1007/s11274-025-04737-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The characterization of halophilic polyhydroxyalkanoate-producing bacteria from brine in Solivar near Prešov (Slovakia)

Popis výsledku v původním jazyce

The present study aims to isolate and investigate temporal variability of the halophilic and halotolerant microbiota present in brine from former salt mine Solivar, Prešov (Slovakia) especially with respect to with their ability to produce polyhydroxyalkanoates (PHA). Brine sampling was performed in the year 2020 and 2021 and samples were inoculated on the R2A medium with 5% NaCl for the bacterial isolation. We obtained a total of 53 halophilic isolates and one halotolerant isolate, all of which were tested for their ability to produce PHA via Nile Blue A staining, Raman spectroscopy and Gas chromatography. The low diverse halophilic microbiota was dominated by Proteobacteria members (mainly Halomonas, Halovibrio, and Chromohalobacter sp.) and some of these bacteria represent newly identified taxa. Around 80% of the isolates were able to produce PHA during growth on glucose-rich media, which highlights the importance of PHA for adaptation to high-salinity environments. Poly(3-hydroxybutyrate) (PHB) was the main type of PHA produced with the yield up to 2.76 g/L in Halovibrio sp. HP20-59. Overall, our investigation pointed out that brine from Solivar shows genetically variable community of halophilic bacteria most of which are capable of accumulation of PHA, hereby confirming the high biotechnological potential of halophilic bacteria.

Název v anglickém jazyce

The characterization of halophilic polyhydroxyalkanoate-producing bacteria from brine in Solivar near Prešov (Slovakia)

Popis výsledku anglicky

The present study aims to isolate and investigate temporal variability of the halophilic and halotolerant microbiota present in brine from former salt mine Solivar, Prešov (Slovakia) especially with respect to with their ability to produce polyhydroxyalkanoates (PHA). Brine sampling was performed in the year 2020 and 2021 and samples were inoculated on the R2A medium with 5% NaCl for the bacterial isolation. We obtained a total of 53 halophilic isolates and one halotolerant isolate, all of which were tested for their ability to produce PHA via Nile Blue A staining, Raman spectroscopy and Gas chromatography. The low diverse halophilic microbiota was dominated by Proteobacteria members (mainly Halomonas, Halovibrio, and Chromohalobacter sp.) and some of these bacteria represent newly identified taxa. Around 80% of the isolates were able to produce PHA during growth on glucose-rich media, which highlights the importance of PHA for adaptation to high-salinity environments. Poly(3-hydroxybutyrate) (PHB) was the main type of PHA produced with the yield up to 2.76 g/L in Halovibrio sp. HP20-59. Overall, our investigation pointed out that brine from Solivar shows genetically variable community of halophilic bacteria most of which are capable of accumulation of PHA, hereby confirming the high biotechnological potential of halophilic bacteria.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

World Journal of Microbiology and Biotechnology

ISSN

0959-3993

e-ISSN

1573-0972

Svazek periodika

41

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

13

Strana od-do

505

Kód UT WoS článku

001637520000001

EID výsledku v databázi Scopus

2-s2.0-105024753372