Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F25%3A00643219" target="_blank" >RIV/68081731:_____/25:00643219 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0144861725003881" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0144861725003881</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carbpol.2025.123607" target="_blank" >10.1016/j.carbpol.2025.123607</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants

Popis výsledku v původním jazyce

Encapsulation of Plant Growth-Promoting Rhizobacteria (PGPR) in hydrogel carriers is a cutting-edge approach in developing agricultural bioinoculants, aiming to improve soil fertility and crop yield. Hydrogels provide protection against environmental stress, though traditional methods using external gel-forming agents limit economic viability. This study presents a novel approach, demonstrating the entrapment of Azotobacter vinelandii, a promising PGPR, within a gel matrix formed by Ca2+-induced crosslinking of its own exopolysaccharide, alginate. Among the five strains evaluated, A. vinelandii DSM 87, DSM 720, and DSM 13529 showed the highest alginate production, peaking at 4.9 +/- 0.6 g/L, 3.5 +/- 0.5 g/L, 3.8 +/- 0.8 g/L, and enabling stable gel formation by the fourth day of cultivation. These strains also exhibited molecular weights and chemical structures of alginate suitable for effective gelation upon Ca2+addition. Additionally, these strains demonstrated significant plant growth-promoting activities, including indole acetic acid production (up to 10.5 mu g/mL), siderophore release, and phosphate solubilization, further validating their potential for sustainable bioinoculant production. Finally, the viability of the A.vinelandii cells released from the gels was experimentally verified. Our findings support a feasible, cost-effective method for bioinoculant production that leverages A. vinelandii's intrinsic capabilities, offering a sustainable alternative to conventional agricultural practices.

Název v anglickém jazyce

Self-entrapment of Azotobacter vinelandii cultures by gelation of their exopolysaccharides: A way towards next-generation bioinoculants

Popis výsledku anglicky

Encapsulation of Plant Growth-Promoting Rhizobacteria (PGPR) in hydrogel carriers is a cutting-edge approach in developing agricultural bioinoculants, aiming to improve soil fertility and crop yield. Hydrogels provide protection against environmental stress, though traditional methods using external gel-forming agents limit economic viability. This study presents a novel approach, demonstrating the entrapment of Azotobacter vinelandii, a promising PGPR, within a gel matrix formed by Ca2+-induced crosslinking of its own exopolysaccharide, alginate. Among the five strains evaluated, A. vinelandii DSM 87, DSM 720, and DSM 13529 showed the highest alginate production, peaking at 4.9 +/- 0.6 g/L, 3.5 +/- 0.5 g/L, 3.8 +/- 0.8 g/L, and enabling stable gel formation by the fourth day of cultivation. These strains also exhibited molecular weights and chemical structures of alginate suitable for effective gelation upon Ca2+addition. Additionally, these strains demonstrated significant plant growth-promoting activities, including indole acetic acid production (up to 10.5 mu g/mL), siderophore release, and phosphate solubilization, further validating their potential for sustainable bioinoculant production. Finally, the viability of the A.vinelandii cells released from the gels was experimentally verified. Our findings support a feasible, cost-effective method for bioinoculant production that leverages A. vinelandii's intrinsic capabilities, offering a sustainable alternative to conventional agricultural practices.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Carbohydrate Polymers

ISSN

0144-8617

e-ISSN

1879-1344

Svazek periodika

360

Číslo periodika v rámci svazku

15 July

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

123607

Kód UT WoS článku

001474799900001

EID výsledku v databázi Scopus

2-s2.0-105002798618