Torpor/hibernation cycle may enhance the risk of insecticides for bats: an in vitro study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F22%3A00555569" target="_blank" >RIV/68081766:_____/22:00555569 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/22:43880362

Výsledek na webu

<a href="https://actavet.vfu.cz/91/1/0059/" target="_blank" >https://actavet.vfu.cz/91/1/0059/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202291010059" target="_blank" >10.2754/avb202291010059</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Torpor/hibernation cycle may enhance the risk of insecticides for bats: an in vitro study

Popis výsledku v původním jazyce

Exposure to pollutants is considered one of the potential reasons of population declines in bats. In the context of previous studies, we managed to create and keep a wide collection of cell lines from European bat species. Liver cells were chosen for testing, as they represent the preferred model for toxicological studies. Bats are protected, cell lines replacing experimental animals thus represent a unique opportunity to examine effects of pollutants which animals are exposed to in their environments. Moreover, cell incubation temperature variation may simulate physiological states of heterothermic bats. Liver cell lines were cultivated to the required cell number. Exposure to five different concentrations of permethrin (PM) and imidacloprid (IMI) were used to determine cytotoxic effects of these pesticides on Nyctalus noctula-derived liver cells cultivated at 37 degrees C and 8 degrees C for 24 h. An assay based on the measurement of activity of lactate dehydrogenase released from damaged cells was used for quantitating cytotoxicity. Cytotoxicity of IMI ranged from 0% to 47% and from 56% to 67% at 37 degrees C and 8 degrees C, respectively. Cytotoxicity of PM ranged from 36% to 56% and from 43% to 88% at 37 degrees C and 8 degrees C, respectively. Permethrin was tested on the cells at an order of magnitude lower concentrations, even so, higher degree of cytotoxicity was recorded. Imidacloprid was more toxic to bat liver cells at a hibernation temperature than at body temperature of 37 degrees C.

Název v anglickém jazyce

Torpor/hibernation cycle may enhance the risk of insecticides for bats: an in vitro study

Popis výsledku anglicky

Exposure to pollutants is considered one of the potential reasons of population declines in bats. In the context of previous studies, we managed to create and keep a wide collection of cell lines from European bat species. Liver cells were chosen for testing, as they represent the preferred model for toxicological studies. Bats are protected, cell lines replacing experimental animals thus represent a unique opportunity to examine effects of pollutants which animals are exposed to in their environments. Moreover, cell incubation temperature variation may simulate physiological states of heterothermic bats. Liver cell lines were cultivated to the required cell number. Exposure to five different concentrations of permethrin (PM) and imidacloprid (IMI) were used to determine cytotoxic effects of these pesticides on Nyctalus noctula-derived liver cells cultivated at 37 degrees C and 8 degrees C for 24 h. An assay based on the measurement of activity of lactate dehydrogenase released from damaged cells was used for quantitating cytotoxicity. Cytotoxicity of IMI ranged from 0% to 47% and from 56% to 67% at 37 degrees C and 8 degrees C, respectively. Cytotoxicity of PM ranged from 36% to 56% and from 43% to 88% at 37 degrees C and 8 degrees C, respectively. Permethrin was tested on the cells at an order of magnitude lower concentrations, even so, higher degree of cytotoxicity was recorded. Imidacloprid was more toxic to bat liver cells at a hibernation temperature than at body temperature of 37 degrees C.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Svazek periodika

91

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

59-68

Kód UT WoS článku

000752524500007

EID výsledku v databázi Scopus

2-s2.0-85124456600