Comparative sperm proteomics in selected passerine birds reflects sperm morphology and mitochondrial metabolism

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F23%3A00577017" target="_blank" >RIV/68081766:_____/23:00577017 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10467743

Výsledek na webu

<a href="https://bioone.org/journalArticle/Download?urlId=10.25225%2Fjvb.23045" target="_blank" >https://bioone.org/journalArticle/Download?urlId=10.25225%2Fjvb.23045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.25225/jvb.23045" target="_blank" >10.25225/jvb.23045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative sperm proteomics in selected passerine birds reflects sperm morphology and mitochondrial metabolism

Popis výsledku v původním jazyce

Spermatozoa are the most variable cells across animal taxa. Phylogeny, speciation and postcopulatory sexual selection are typical factors that explain the sperm morphology variation in animals, and now these differences can also be explored on the level of genomic and proteomic differentiation. However, in non-model organisms, it is often difficult to employ these techniques because genomes are not yet available for most animal species, particularly for free-living songbirds (Passeriformes). Here, we employed label-free proteomics to generate proteomes in the zebra finch, a songbird species with an annotated genome and five wild-living songbirds representing five families within the Passerida clade, all with poorly known genomes. The results show that protein mapping of the new passerine proteomes to the zebra finch genome was successful, thus yielding highly similar protein identifications and a sufficient number of unique peptides in all the studied proteomes. Interestingly, while passerine sperm proteomes only partially reflect phylogenetic relationships between passerine families, midpiece length correlates with at least 59 proteins enriched in mitochondrial metabolism. Similar sperm proteomes seem to have evolved convergently across passerine lineages, potentially due to varying levels of sperm competition and marked variation in sperm sizes.

Název v anglickém jazyce

Comparative sperm proteomics in selected passerine birds reflects sperm morphology and mitochondrial metabolism

Popis výsledku anglicky

Spermatozoa are the most variable cells across animal taxa. Phylogeny, speciation and postcopulatory sexual selection are typical factors that explain the sperm morphology variation in animals, and now these differences can also be explored on the level of genomic and proteomic differentiation. However, in non-model organisms, it is often difficult to employ these techniques because genomes are not yet available for most animal species, particularly for free-living songbirds (Passeriformes). Here, we employed label-free proteomics to generate proteomes in the zebra finch, a songbird species with an annotated genome and five wild-living songbirds representing five families within the Passerida clade, all with poorly known genomes. The results show that protein mapping of the new passerine proteomes to the zebra finch genome was successful, thus yielding highly similar protein identifications and a sufficient number of unique peptides in all the studied proteomes. Interestingly, while passerine sperm proteomes only partially reflect phylogenetic relationships between passerine families, midpiece length correlates with at least 59 proteins enriched in mitochondrial metabolism. Similar sperm proteomes seem to have evolved convergently across passerine lineages, potentially due to varying levels of sperm competition and marked variation in sperm sizes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10613 - Zoology

Projekt

<a href="/cs/project/GA19-22538S" target="_blank" >GA19-22538S: Molekulární mechanismy morfologické variability spermií pěvců</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Vertebrate Biology

ISSN

2694-7684

e-ISSN

2694-7684

Svazek periodika

72

Číslo periodika v rámci svazku

23045

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

14

Strana od-do

23045

Kód UT WoS článku

001067624800001

EID výsledku v databázi Scopus

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