Diversity of filariae circulating in South Caucasian bats and their ectoparasites

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F25%3A00638515" target="_blank" >RIV/68081766:_____/25:00638515 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/25:102420 RIV/00216224:14310/25:00142271 RIV/62157124:16270/25:43882610

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2667114X25000640?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2667114X25000640?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.crpvbd.2025.100304" target="_blank" >10.1016/j.crpvbd.2025.100304</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diversity of filariae circulating in South Caucasian bats and their ectoparasites

Popis výsledku v původním jazyce

Recent research on chiropteran parasites suggests a high prevalence and diversity, and extensive spatial distribution of filarial species, however, ecological and phylogenetic studies are still in their infancy. We sampled blood from 78 bat specimens, collected 1181 ectoparasites at summer colonies in Armenia and Georgia, and used nested-PCR targeting the cytochrome c oxidase subunit 1 (cox1) gene to detect and genotype filarial parasites. The overall prevalence of filarial DNA was 17.9% in blood samples from Myotis blythii, Myotis emarginatus, Miniopterus schreibersii, and Rhinolophus ferrumequinum, and 8.5% in ectoparasites, including two mite species (Eyndhovenia euryalis and Spinturnix myoti) and two bat flies (Nycteribia kolenatii and Penicillidia dufouri). The prevalence of microfilarial infection was significantly higher in mite samples (13.8%) than in bat fly samples (4.1%). Bats with ectoparasites positive for filarial DNA had a significantly higher total number of ectoparasites. Phylogenetic analysis placed the 18 sequences obtained into different closely related clades of onchocercid nematodes, with four different species recorded: two belonging to the genus Litomosa and two to a newly observed genus of the family Onchocercidae. Additionally, two new species of these parasites, one Litomosa sp. and one Onchocercid sp., were genetically recognised. As predicted, the diversity of filarial parasites reflects the diversity of bat hosts in the Caucasus. Extending the sampling effort to more Caucasian bat species will likely reveal previously unknown filarial species. Non-lethal and non-invasive sampling of blood and ectoparasites for molecular screening proved effective for gaining insights into parasite diversity and phylogenetic relationships of bat-infecting filarial nematodes.

Název v anglickém jazyce

Diversity of filariae circulating in South Caucasian bats and their ectoparasites

Popis výsledku anglicky

Recent research on chiropteran parasites suggests a high prevalence and diversity, and extensive spatial distribution of filarial species, however, ecological and phylogenetic studies are still in their infancy. We sampled blood from 78 bat specimens, collected 1181 ectoparasites at summer colonies in Armenia and Georgia, and used nested-PCR targeting the cytochrome c oxidase subunit 1 (cox1) gene to detect and genotype filarial parasites. The overall prevalence of filarial DNA was 17.9% in blood samples from Myotis blythii, Myotis emarginatus, Miniopterus schreibersii, and Rhinolophus ferrumequinum, and 8.5% in ectoparasites, including two mite species (Eyndhovenia euryalis and Spinturnix myoti) and two bat flies (Nycteribia kolenatii and Penicillidia dufouri). The prevalence of microfilarial infection was significantly higher in mite samples (13.8%) than in bat fly samples (4.1%). Bats with ectoparasites positive for filarial DNA had a significantly higher total number of ectoparasites. Phylogenetic analysis placed the 18 sequences obtained into different closely related clades of onchocercid nematodes, with four different species recorded: two belonging to the genus Litomosa and two to a newly observed genus of the family Onchocercidae. Additionally, two new species of these parasites, one Litomosa sp. and one Onchocercid sp., were genetically recognised. As predicted, the diversity of filarial parasites reflects the diversity of bat hosts in the Caucasus. Extending the sampling effort to more Caucasian bat species will likely reveal previously unknown filarial species. Non-lethal and non-invasive sampling of blood and ectoparasites for molecular screening proved effective for gaining insights into parasite diversity and phylogenetic relationships of bat-infecting filarial nematodes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Research in Parasitology and Vector-Borne Diseases

ISSN

2667-114X

e-ISSN

2667-114X

Svazek periodika

8

Číslo periodika v rámci svazku

100304

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

100304

Kód UT WoS článku

001555781900001

EID výsledku v databázi Scopus

2-s2.0-105013152729