Psychrotolerant enterobacters inhabiting the gut of Antarctic fishes of the family Nototheniidae and description of Enterobacter hoffmannii subsp. nototheniae subsp. nov.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F25%3A00644487" target="_blank" >RIV/68081766:_____/25:00644487 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00143229

Výsledek na webu

<a href="https://journals.muni.cz/CPR/article/view/42792" target="_blank" >https://journals.muni.cz/CPR/article/view/42792</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5817/CPR2025-2-14" target="_blank" >10.5817/CPR2025-2-14</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Psychrotolerant enterobacters inhabiting the gut of Antarctic fishes of the family Nototheniidae and description of Enterobacter hoffmannii subsp. nototheniae subsp. nov.

Popis výsledku v původním jazyce

The gut microbiota of four Antarctic marine fish species – Notothenia coriiceps, Trematomus bernacchii, Trematomus hansoni, and Trematomus newnesi – were analysed, with a particular focus on the members of the Enterobacter cloacae complex. Nineteen isolated strains were characterised by rep-PCR, automated ribotyping, extended phenotyping, and MALDI-TOF MS analysis. Fingerprinting methods grouped the psychrotolerant isolates into two distinct groups, representing the dominant enteric bacteria in the gut contents of these fish. Following their preliminary identification as members of the Enterobacter cloacae complex, additional phylogenetic analyses were conducted using the 16S rRNA and rpoB genes, as well as fatty acid analysis and wholegenome sequencing of two representatives selected based on their fingerprints. All results indicated that the analysed group represents a new autochthonous Enterobacter taxon inhabiting the gut of fish of the family Nototheniidae. Based on average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values, the “Enterobacter hoffmannii”, the effectively published but non-valid name, was the most closely relatednspecies to representatives of both groups of isolates from the gut of the above-specified notothenioid fish. The name Enterobacter hoffmannii subsp. nototheniae subsp. nov. is proposed, with the type strain P5473T (= CCM 8629T = LMG 34031T), for isolates of Group 1. At the same time, the name Enterobacter hoffmannii subsp. hoffmannii subsp. nov. is suggested for isolates of Group 2, with the existing type strain DSM 14563T = LMG 30171T.

Název v anglickém jazyce

Psychrotolerant enterobacters inhabiting the gut of Antarctic fishes of the family Nototheniidae and description of Enterobacter hoffmannii subsp. nototheniae subsp. nov.

Popis výsledku anglicky

The gut microbiota of four Antarctic marine fish species – Notothenia coriiceps, Trematomus bernacchii, Trematomus hansoni, and Trematomus newnesi – were analysed, with a particular focus on the members of the Enterobacter cloacae complex. Nineteen isolated strains were characterised by rep-PCR, automated ribotyping, extended phenotyping, and MALDI-TOF MS analysis. Fingerprinting methods grouped the psychrotolerant isolates into two distinct groups, representing the dominant enteric bacteria in the gut contents of these fish. Following their preliminary identification as members of the Enterobacter cloacae complex, additional phylogenetic analyses were conducted using the 16S rRNA and rpoB genes, as well as fatty acid analysis and wholegenome sequencing of two representatives selected based on their fingerprints. All results indicated that the analysed group represents a new autochthonous Enterobacter taxon inhabiting the gut of fish of the family Nototheniidae. Based on average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values, the “Enterobacter hoffmannii”, the effectively published but non-valid name, was the most closely relatednspecies to representatives of both groups of isolates from the gut of the above-specified notothenioid fish. The name Enterobacter hoffmannii subsp. nototheniae subsp. nov. is proposed, with the type strain P5473T (= CCM 8629T = LMG 34031T), for isolates of Group 1. At the same time, the name Enterobacter hoffmannii subsp. hoffmannii subsp. nov. is suggested for isolates of Group 2, with the existing type strain DSM 14563T = LMG 30171T.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Polar Reports

ISSN

1805-0689

e-ISSN

1805-0697

Svazek periodika

15

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

25

Strana od-do

224-248

Kód UT WoS článku

001663035800004

EID výsledku v databázi Scopus

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