Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F13%3A00398237" target="_blank" >RIV/68378041:_____/13:00398237 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bbrc.2013.09.133" target="_blank" >http://dx.doi.org/10.1016/j.bbrc.2013.09.133</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbrc.2013.09.133" target="_blank" >10.1016/j.bbrc.2013.09.133</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS

Popis výsledku v původním jazyce

ELISA is widely used for urinary 8-oxo-7.8-dihydro-2´-deoxyguanosine (8-oxodG) analysis. However, a lack of agreement between ELISA and chromatographic methods confines its application to the assessment of relative urinary 8-oxodG level. We investigatedvarious ELISA modifications, seeking optimal conditions that would yield a good agreement between ELISA and HPLC-MS/MS. It was found, that purification of urine by solid phase extraction, then incubation with the anti-8-oxodG antibody at 40 C overnight and subsequent normalization of 8-oxodG levels per urinary creatinine resulted in a near-perfect correlation and agreement in mean levels between ELISA and HPLC-MS/MS. Our data show that, after introduction of a simple modification, ELISA quantification urinary 8-oxodG substantially improves. This method represents a significant advance for the ELISA, and encouraging its use in more studies adding to our knowledge of the role of this biomarker of oxidative stress in health and disease.

Název v anglickém jazyce

Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS

Popis výsledku anglicky

ELISA is widely used for urinary 8-oxo-7.8-dihydro-2´-deoxyguanosine (8-oxodG) analysis. However, a lack of agreement between ELISA and chromatographic methods confines its application to the assessment of relative urinary 8-oxodG level. We investigatedvarious ELISA modifications, seeking optimal conditions that would yield a good agreement between ELISA and HPLC-MS/MS. It was found, that purification of urine by solid phase extraction, then incubation with the anti-8-oxodG antibody at 40 C overnight and subsequent normalization of 8-oxodG levels per urinary creatinine resulted in a near-perfect correlation and agreement in mean levels between ELISA and HPLC-MS/MS. Our data show that, after introduction of a simple modification, ELISA quantification urinary 8-oxodG substantially improves. This method represents a significant advance for the ELISA, and encouraging its use in more studies adding to our knowledge of the role of this biomarker of oxidative stress in health and disease.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical and Biophysical Research Communications

ISSN

0006-291X

e-ISSN

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Svazek periodika

440

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

725-730

Kód UT WoS článku

000326905800045

EID výsledku v databázi Scopus

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