Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F15%3A00449670" target="_blank" >RIV/68378041:_____/15:00449670 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ceca.2014.11.003" target="_blank" >http://dx.doi.org/10.1016/j.ceca.2014.11.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ceca.2014.11.003" target="_blank" >10.1016/j.ceca.2014.11.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus

Popis výsledku v původním jazyce

The purpose of the present study was to uncover the structure and function of molecules related to the central osmoreceptorin the supraoptic nucleus (SON) of rats. For this purpose, we performed RT-PCR and immunohistochemistry for TRPV1-related molecules, and patch-clamp and imaging of the cytosolic Ca2+ concentration ([Ca2+]i) to measure responses to osmolality changes and TRPV-related drugs. Arginine vasopressin (AVP), synthesized in the SON is a key factor in systemic osmolality regulation. We foundthat AVP neurones in the SON possess functional full-length TRPV1. Moreover, differences between the responses to capsaicin or hyperosmolality obtained in rat SON neurones and those obtained from dorsal root ganglion neurones or TRPV1-expressing cells indicate that the osmoreceptor expressed in the SON may be a heteromultimer in which TRPV1 is co-assembled with some other, yet unidentified, molecules.

Název v anglickém jazyce

Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus

Popis výsledku anglicky

The purpose of the present study was to uncover the structure and function of molecules related to the central osmoreceptorin the supraoptic nucleus (SON) of rats. For this purpose, we performed RT-PCR and immunohistochemistry for TRPV1-related molecules, and patch-clamp and imaging of the cytosolic Ca2+ concentration ([Ca2+]i) to measure responses to osmolality changes and TRPV-related drugs. Arginine vasopressin (AVP), synthesized in the SON is a key factor in systemic osmolality regulation. We foundthat AVP neurones in the SON possess functional full-length TRPV1. Moreover, differences between the responses to capsaicin or hyperosmolality obtained in rat SON neurones and those obtained from dorsal root ganglion neurones or TRPV1-expressing cells indicate that the osmoreceptor expressed in the SON may be a heteromultimer in which TRPV1 is co-assembled with some other, yet unidentified, molecules.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Calcium

ISSN

0143-4160

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

13

Strana od-do

24-37

Kód UT WoS článku

000349574000003

EID výsledku v databázi Scopus

2-s2.0-84922644005