Differentiation potential of neonatal neural stem/progenitor cells is affected by WNT signaling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F15%3A00455570" target="_blank" >RIV/68378041:_____/15:00455570 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/15:00455570

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Differentiation potential of neonatal neural stem/progenitor cells is affected by WNT signaling

Popis výsledku v původním jazyce

The canonical Wnt signaling pathway plays an important role in embryogenesis and in establishment of neurogenic niches. Studies using embryonic and neonatal mice suggested involvement of Wnt/beta-catenin signaling in proliferation and differentiation ofneural progenitors. The effect of Wnt signaling on the differentiation potential of neonatal neural stem/progenitor cells (NS/PCs) and their membrane properties was studied eight days after the onset of in vitro differentiation. To manipulate Wnt signaling at different subcellular levels, three transgenic mouse strains were employed, which enabled either inhibition of the pathway at the nuclear or cell membrane level, or activation of Wnt signaling in the cytoplasm using the Cre-loxP system. To follow the fate of NS/PCs, the patch-clamp technique, immunocytochemistry, calcium imaging, Western blot, and quantitative RT-PCR (RT-qPCR) was utilized. Within differentiated cells we found three electrophysiologically and immunocytochemically d

Název v anglickém jazyce

Differentiation potential of neonatal neural stem/progenitor cells is affected by WNT signaling

Popis výsledku anglicky

The canonical Wnt signaling pathway plays an important role in embryogenesis and in establishment of neurogenic niches. Studies using embryonic and neonatal mice suggested involvement of Wnt/beta-catenin signaling in proliferation and differentiation ofneural progenitors. The effect of Wnt signaling on the differentiation potential of neonatal neural stem/progenitor cells (NS/PCs) and their membrane properties was studied eight days after the onset of in vitro differentiation. To manipulate Wnt signaling at different subcellular levels, three transgenic mouse strains were employed, which enabled either inhibition of the pathway at the nuclear or cell membrane level, or activation of Wnt signaling in the cytoplasm using the Cre-loxP system. To follow the fate of NS/PCs, the patch-clamp technique, immunocytochemistry, calcium imaging, Western blot, and quantitative RT-PCR (RT-qPCR) was utilized. Within differentiated cells we found three electrophysiologically and immunocytochemically d

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP303%2F12%2F0855" target="_blank" >GAP303/12/0855: Polydendrocyty a regenerace ischemicky poškozeného mozku - úloha Wnt signalizační dráhy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů