Human multipotent mesenchymal stromal cells in the treatment of postoperative temporal bone defect: an animal model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F16%3A00450151" target="_blank" >RIV/68378041:_____/16:00450151 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/16:10320100 RIV/00216208:11150/16:10320100 RIV/00179906:_____/16:10320100

Výsledek na webu

<a href="http://dx.doi.org/10.3727/096368915X689730" target="_blank" >http://dx.doi.org/10.3727/096368915X689730</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3727/096368915X689730" target="_blank" >10.3727/096368915X689730</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Human multipotent mesenchymal stromal cells in the treatment of postoperative temporal bone defect: an animal model

Popis výsledku v původním jazyce

Canal wall down mastoidectomy is one of the most effective treatments for cholesteatoma. However, it results in anatomical changes in the external and middle ear with a negative impact on the patient's quality of life. To provide complete closure of the mastoid cavity and normalize the anatomy of the middle and external ear, we used human multipotent mesenchymal stromal cells (hMSCs), GMP grade, in a guinea pig model. A method for preparing a biomaterial composed of hMSCs, hydroxyapatite, and tissue glue was developed. Animals from the treated group were implanted with biomaterial composed of hydroxyapatite and hMSCs, while animals in the control group received hydroxyapatite alone. When compared to controls, the group implanted with hMSCs showed a significantly higher ratio of new bone formation (p = 0.00174), as well as a significantly higher volume percentage of new immature bone (p = 0.00166). Our results proved a beneficial effect of hMSCs on temporal bone formation and provided a promising tool to improve the quality of life of patients after canal wall down mastoidectomy by hMSC implantation.

Název v anglickém jazyce

Human multipotent mesenchymal stromal cells in the treatment of postoperative temporal bone defect: an animal model

Popis výsledku anglicky

Canal wall down mastoidectomy is one of the most effective treatments for cholesteatoma. However, it results in anatomical changes in the external and middle ear with a negative impact on the patient's quality of life. To provide complete closure of the mastoid cavity and normalize the anatomy of the middle and external ear, we used human multipotent mesenchymal stromal cells (hMSCs), GMP grade, in a guinea pig model. A method for preparing a biomaterial composed of hMSCs, hydroxyapatite, and tissue glue was developed. Animals from the treated group were implanted with biomaterial composed of hydroxyapatite and hMSCs, while animals in the control group received hydroxyapatite alone. When compared to controls, the group implanted with hMSCs showed a significantly higher ratio of new bone formation (p = 0.00174), as well as a significantly higher volume percentage of new immature bone (p = 0.00166). Our results proved a beneficial effect of hMSCs on temporal bone formation and provided a promising tool to improve the quality of life of patients after canal wall down mastoidectomy by hMSC implantation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

—

Projekt

<a href="/cs/project/LO1309" target="_blank" >LO1309: Buněčná terapie a tkáňové náhrady</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Transplantation

ISSN

0963-6897

e-ISSN

—

Svazek periodika

25

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

28

Strana od-do

1405-1414

Kód UT WoS článku

000384801200017

EID výsledku v databázi Scopus

2-s2.0-84978134221