High throughput toxicity screening and intracellular detection of nanomaterials.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00469950" target="_blank" >RIV/68378041:_____/17:00469950 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/wnan.1413" target="_blank" >http://dx.doi.org/10.1002/wnan.1413</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/wnan.1413" target="_blank" >10.1002/wnan.1413</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High throughput toxicity screening and intracellular detection of nanomaterials.

Popis výsledku v původním jazyce

With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safetypreferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read-across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter-experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM-cell interactions. Validation of in vitroHTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose- and time-dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label-free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance-based monitoring, Multiplex analysis of secreted products, and genotoxicity methodsnamely High throughput comet assay, High throughput in vitro micronucleus assay, and H2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test, possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed.

Název v anglickém jazyce

High throughput toxicity screening and intracellular detection of nanomaterials.

Popis výsledku anglicky

With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safetypreferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read-across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter-experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM-cell interactions. Validation of in vitroHTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose- and time-dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label-free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance-based monitoring, Multiplex analysis of secreted products, and genotoxicity methodsnamely High throughput comet assay, High throughput in vitro micronucleus assay, and H2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test, possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30304 - Public and environmental health

Projekt

<a href="/cs/project/LD14002" target="_blank" >LD14002: Toxické účinky nanomateriálů jako funkce jejich struktury a fyzikálně-chemických vlastností</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology

ISSN

1939-5116

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

30

Strana od-do

—

Kód UT WoS článku

000392508900006

EID výsledku v databázi Scopus

2-s2.0-85006488641