Brain Diffusivity and Structural Changes in the R6/2 Mouse Model of Huntington Disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00476755" target="_blank" >RIV/68378041:_____/17:00476755 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/17:10373797 RIV/00159816:_____/17:00068491

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jnr.23965" target="_blank" >http://dx.doi.org/10.1002/jnr.23965</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jnr.23965" target="_blank" >10.1002/jnr.23965</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Brain Diffusivity and Structural Changes in the R6/2 Mouse Model of Huntington Disease

Popis výsledku v původním jazyce

Diffusion-weighted magnetic resonance (DW-MR) is an important diagnostic tool in Huntington disease (HD), a fatal hereditary neurodegenerative disorder. To clarify the nature of diffusivity changes in HD, we compared the apparent diffusion coefficient of water (ADC(W)) acquired by DW-MR with extracellular space volume fraction a and tortuosity lambda, measured by the iontophoretic method in the R6/2 mouse model of HD and in wild-type controls (WT). In anisotropic globus pallidus (GP), diffusion measurements were performed in the mediolateral (x), rostrocaudal (y), and ventrodorsal (z) axes. In HD animals, we detected an increase in ADCW in all axes and larger a than in WT mice. No significant difference between WT and HD mice was found in the values of tortuosity (lambda(x), lambda(y), lambda(z)). Despite structural changes in GP, diffusion anisotropy was unaffected in HD mice. Immunohistochemical analysis revealed in HD mice weaker expression of extracellular matrix and a decrease in neuron numbers compared with WT mice. Glial fibrillary acidic protein staining detected astrogliosis-like changes in the morphology of astrocytic processes in HD GP. In the somatosensory cortex, no significant differences in the studied parameters were found. We conclude that in the R6/2 model of HD, a decrease in the number of neurons in the GP results in increased ADC(W) and alpha values. Values of lambda were not significantly changed as the increase of diffusion obstacles formed by reactive astrocytes was compensated for by the extracellular matrix reduction.

Název v anglickém jazyce

Brain Diffusivity and Structural Changes in the R6/2 Mouse Model of Huntington Disease

Popis výsledku anglicky

Diffusion-weighted magnetic resonance (DW-MR) is an important diagnostic tool in Huntington disease (HD), a fatal hereditary neurodegenerative disorder. To clarify the nature of diffusivity changes in HD, we compared the apparent diffusion coefficient of water (ADC(W)) acquired by DW-MR with extracellular space volume fraction a and tortuosity lambda, measured by the iontophoretic method in the R6/2 mouse model of HD and in wild-type controls (WT). In anisotropic globus pallidus (GP), diffusion measurements were performed in the mediolateral (x), rostrocaudal (y), and ventrodorsal (z) axes. In HD animals, we detected an increase in ADCW in all axes and larger a than in WT mice. No significant difference between WT and HD mice was found in the values of tortuosity (lambda(x), lambda(y), lambda(z)). Despite structural changes in GP, diffusion anisotropy was unaffected in HD mice. Immunohistochemical analysis revealed in HD mice weaker expression of extracellular matrix and a decrease in neuron numbers compared with WT mice. Glial fibrillary acidic protein staining detected astrogliosis-like changes in the morphology of astrocytic processes in HD GP. In the somatosensory cortex, no significant differences in the studied parameters were found. We conclude that in the R6/2 model of HD, a decrease in the number of neurons in the GP results in increased ADC(W) and alpha values. Values of lambda were not significantly changed as the increase of diffusion obstacles formed by reactive astrocytes was compensated for by the extracellular matrix reduction.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Neuroscience Research

ISSN

0360-4012

e-ISSN

—

Svazek periodika

95

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

1474-1484

Kód UT WoS článku

000402441800011

EID výsledku v databázi Scopus

2-s2.0-85006350370