Perfusion bioreactor-based cryopreservation of 3D human mesenchymal stromal cell tissue grafts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00486485" target="_blank" >RIV/68378041:_____/17:00486485 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cryobiol.2017.04.001" target="_blank" >http://dx.doi.org/10.1016/j.cryobiol.2017.04.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cryobiol.2017.04.001" target="_blank" >10.1016/j.cryobiol.2017.04.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Perfusion bioreactor-based cryopreservation of 3D human mesenchymal stromal cell tissue grafts

Popis výsledku v původním jazyce

The generation of an off-the-shelf in vitro engineered living tissue graft will likely require cryopreservation. However, the efficient addition and removal of cryoprotective agents (CPA) to cells throughout the volume of a three-dimensional (3D) tissue graft remains a significant challenge. In this work, we assessed whether a perfusion bioreactor-based method could be used to improve the viability of cryopreserved mesenchymal stromal cell- (MSC) based tissue constructs as compared to using conventional diffusion-based methods. The bioreactor was first used to saturate 3D constructs with CPA under perfused flow. Following cryopreservation, the bioreactor was then also used for the efficient removal of the CPA from the 3D tissues. We demonstrate that addition and removal of CPA under perfused flow significantly increased the viability of MSC within cryopreserved 3D tissue constructs as compared to conventional diffusion-based methods. (C) 2017 Elsevier Inc. All rights reserved.

Název v anglickém jazyce

Perfusion bioreactor-based cryopreservation of 3D human mesenchymal stromal cell tissue grafts

Popis výsledku anglicky

The generation of an off-the-shelf in vitro engineered living tissue graft will likely require cryopreservation. However, the efficient addition and removal of cryoprotective agents (CPA) to cells throughout the volume of a three-dimensional (3D) tissue graft remains a significant challenge. In this work, we assessed whether a perfusion bioreactor-based method could be used to improve the viability of cryopreserved mesenchymal stromal cell- (MSC) based tissue constructs as compared to using conventional diffusion-based methods. The bioreactor was first used to saturate 3D constructs with CPA under perfused flow. Following cryopreservation, the bioreactor was then also used for the efficient removal of the CPA from the 3D tissues. We demonstrate that addition and removal of CPA under perfused flow significantly increased the viability of MSC within cryopreserved 3D tissue constructs as compared to conventional diffusion-based methods. (C) 2017 Elsevier Inc. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cryobiology

ISSN

0011-2240

e-ISSN

—

Svazek periodika

76

Číslo periodika v rámci svazku

jun.

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

150-153

Kód UT WoS článku

000403193400020

EID výsledku v databázi Scopus

2-s2.0-85017228290