The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00518846" target="_blank" >RIV/68378041:_____/19:00518846 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216305:26620/19:PU130004 RIV/00216224:14310/19:00108936
Výsledek na webu
<a href="https://onlinelibrary.wiley.com/doi/abs/10.1111/jmi.12771" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1111/jmi.12771</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jmi.12771" target="_blank" >10.1111/jmi.12771</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography
Popis výsledku v původním jazyce
This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent - heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.
Název v anglickém jazyce
The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography
Popis výsledku anglicky
This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent - heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30404 - Biomaterials (as related to medical implants, devices, sensors)
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Microscopy
ISSN
0022-2720
e-ISSN
—
Svazek periodika
273
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
9
Strana od-do
169-177
Kód UT WoS článku
000458426100002
EID výsledku v databázi Scopus
2-s2.0-85057052831