Decoding the Transcriptional Response to Ischemic Stroke in Young and Aged Mouse Brain

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F20%3A00525487" target="_blank" >RIV/68378041:_____/20:00525487 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/20:00525487 RIV/00216208:11130/20:10412359 RIV/61989592:15310/20:73605171

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2211124720307579?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2211124720307579?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.celrep.2020.107777" target="_blank" >10.1016/j.celrep.2020.107777</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Decoding the Transcriptional Response to Ischemic Stroke in Young and Aged Mouse Brain

Popis výsledku v původním jazyce

Ischemic stroke is a well-recognized disease of aging, yet it is unclear how the age-dependent vulnerability occurs and what are the underlying mechanisms. To address these issues, we perform a comprehensive RNA-seq analysis of aging, ischemic stroke, and their interaction in 3- and 18-month-old mice. We assess differential gene expression across injury status and age, estimate cell type proportion changes, assay the results against a range of transcriptional signatures from the literature, and perform unsupervised co-expression analysis, identifying modules of genes with varying response to injury. We uncover downregulation of axonal and synaptic maintenance genetic program, and increased activation of type I interferon (IFN-I) signaling following stroke in aged mice. Together, these results paint a picture of ischemic stroke as a complex age-related disease and provide insights into interaction of aging and stroke on cellular and molecular level.

Název v anglickém jazyce

Decoding the Transcriptional Response to Ischemic Stroke in Young and Aged Mouse Brain

Popis výsledku anglicky

Ischemic stroke is a well-recognized disease of aging, yet it is unclear how the age-dependent vulnerability occurs and what are the underlying mechanisms. To address these issues, we perform a comprehensive RNA-seq analysis of aging, ischemic stroke, and their interaction in 3- and 18-month-old mice. We assess differential gene expression across injury status and age, estimate cell type proportion changes, assay the results against a range of transcriptional signatures from the literature, and perform unsupervised co-expression analysis, identifying modules of genes with varying response to injury. We uncover downregulation of axonal and synaptic maintenance genetic program, and increased activation of type I interferon (IFN-I) signaling following stroke in aged mice. Together, these results paint a picture of ischemic stroke as a complex age-related disease and provide insights into interaction of aging and stroke on cellular and molecular level.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Reports

ISSN

2211-1247

e-ISSN

—

Svazek periodika

31

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

27

Strana od-do

107777

Kód UT WoS článku

000541973800019

EID výsledku v databázi Scopus

2-s2.0-85086381872