Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F20%3A00539261" target="_blank" >RIV/68378041:_____/20:00539261 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41596-020-0398-1" target="_blank" >https://www.nature.com/articles/s41596-020-0398-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41596-020-0398-1" target="_blank" >10.1038/s41596-020-0398-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Popis výsledku v původním jazyce

The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between desirable and essential information: essential information refers to the precise details that are necessary to assess the quality of the experimental work, whereas desirable information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.nnHere, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).

Název v anglickém jazyce

Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Popis výsledku anglicky

The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between desirable and essential information: essential information refers to the precise details that are necessary to assess the quality of the experimental work, whereas desirable information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.nnHere, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Protocols

ISSN

1754-2189

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

3817-3826

Kód UT WoS článku

000583953500001

EID výsledku v databázi Scopus

2-s2.0-85093963487