Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F21%3A00552873" target="_blank" >RIV/68378041:_____/21:00552873 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/21:00555019

Výsledek na webu

<a href="https://www.nature.com/articles/s41556-021-00746-2" target="_blank" >https://www.nature.com/articles/s41556-021-00746-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41556-021-00746-2" target="_blank" >10.1038/s41556-021-00746-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs

Popis výsledku v původním jazyce

A set of three papers reports that the piRNA pathway is essential for mammalian female fertility based on genetic perturbation experiments performed in golden hamsters.nnPIWI-interacting RNAs (piRNAs) support the germline by suppressing retrotransposons. Studies of the pathway in mice have strongly shaped the view that mammalian piRNAs are essential for male but not for female fertility. Here, we report that the role of the piRNA pathway substantially differs in golden hamsters (Mesocricetus auratus), the piRNA pathway setup of which more closely resembles that of other mammals, including humans. The loss of the Mov10l1 RNA helicase-an essential piRNA biogenesis factor-leads to striking phenotypes in both sexes. In contrast to mice, female Mov10l1(-/-) hamsters are sterile because their oocytes do not sustain zygotic development. Furthermore, Mov10l1(-/-) male hamsters have impaired establishment of spermatogonia accompanied by transcriptome dysregulation and an expression surge of a young retrotransposon subfamily. Our results show that the mammalian piRNA pathway has essential roles in both sexes and its adaptive nature allows it to manage emerging genomic threats and acquire new critical roles in the germline.

Název v anglickém jazyce

Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs

Popis výsledku anglicky

A set of three papers reports that the piRNA pathway is essential for mammalian female fertility based on genetic perturbation experiments performed in golden hamsters.nnPIWI-interacting RNAs (piRNAs) support the germline by suppressing retrotransposons. Studies of the pathway in mice have strongly shaped the view that mammalian piRNAs are essential for male but not for female fertility. Here, we report that the role of the piRNA pathway substantially differs in golden hamsters (Mesocricetus auratus), the piRNA pathway setup of which more closely resembles that of other mammals, including humans. The loss of the Mov10l1 RNA helicase-an essential piRNA biogenesis factor-leads to striking phenotypes in both sexes. In contrast to mice, female Mov10l1(-/-) hamsters are sterile because their oocytes do not sustain zygotic development. Furthermore, Mov10l1(-/-) male hamsters have impaired establishment of spermatogonia accompanied by transcriptome dysregulation and an expression surge of a young retrotransposon subfamily. Our results show that the mammalian piRNA pathway has essential roles in both sexes and its adaptive nature allows it to manage emerging genomic threats and acquire new critical roles in the germline.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Cell Biology

ISSN

1465-7392

e-ISSN

1476-4679

Svazek periodika

23

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

26

Strana od-do

992-1001

Kód UT WoS článku

000692985400002

EID výsledku v databázi Scopus

2-s2.0-85114647881