Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F23%3A00571245" target="_blank" >RIV/68378041:_____/23:00571245 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/23:00571245 RIV/00216208:11130/23:10459037 RIV/00216208:11310/23:10459037 RIV/60461373:22310/23:43928170

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-023-33608-y" target="_blank" >https://www.nature.com/articles/s41598-023-33608-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-023-33608-y" target="_blank" >10.1038/s41598-023-33608-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology

Popis výsledku v původním jazyce

The role of glia in amyotrophic lateral sclerosis (ALS) is undeniable. Their disease-related activity has been extensively studied in the spinal cord, but only partly in the brain. We present herein a comprehensive study of glia in the cortex of SOD1(G93A) mice—a widely used model of ALS. Using single-cell RNA sequencing (scRNA-seq) and immunohistochemistry, we inspected astrocytes, microglia, and oligodendrocytes, in four stages of the disease, respecting the factor of sex. We report minimal changes of glia throughout the disease progression and regardless of sex. Pseudobulk and single-cell analyses revealed subtle disease-related transcriptional alterations at the end-stage in microglia and oligodendrocytes, which were supported by immunohistochemistry. Therefore, our data support the hypothesis that the SOD1(G93A) mouse cortex does not recapitulate the disease in patients, and we recommend the use of a different model for future studies of the cortical ALS pathology.

Název v anglickém jazyce

Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology

Popis výsledku anglicky

The role of glia in amyotrophic lateral sclerosis (ALS) is undeniable. Their disease-related activity has been extensively studied in the spinal cord, but only partly in the brain. We present herein a comprehensive study of glia in the cortex of SOD1(G93A) mice—a widely used model of ALS. Using single-cell RNA sequencing (scRNA-seq) and immunohistochemistry, we inspected astrocytes, microglia, and oligodendrocytes, in four stages of the disease, respecting the factor of sex. We report minimal changes of glia throughout the disease progression and regardless of sex. Pseudobulk and single-cell analyses revealed subtle disease-related transcriptional alterations at the end-stage in microglia and oligodendrocytes, which were supported by immunohistochemistry. Therefore, our data support the hypothesis that the SOD1(G93A) mouse cortex does not recapitulate the disease in patients, and we recommend the use of a different model for future studies of the cortical ALS pathology.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

13

Číslo periodika v rámci svazku

april

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

6538

Kód UT WoS článku

000989734900016

EID výsledku v databázi Scopus

2-s2.0-85153548229