Protamine sequence determines species-specific nuclear shape and histone retention

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F25%3A00637862" target="_blank" >RIV/68378041:_____/25:00637862 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027014:_____/25:10006339

Výsledek na webu

<a href="https://www.cell.com/iscience/fulltext/S2589-0042(25)01363-X" target="_blank" >https://www.cell.com/iscience/fulltext/S2589-0042(25)01363-X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.isci.2025.113102" target="_blank" >10.1016/j.isci.2025.113102</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Protamine sequence determines species-specific nuclear shape and histone retention

Popis výsledku v původním jazyce

The nuclear shape observed after the forced expression of mouse or human protamine 1 (PRM1) in fibroblasts led us to propose the hypothesis that the PRM1 sequence plays an important role in imposing the overall shape of the protaminized nucleus. Comparison of mouse and human PRM1 sequences pointed to cysteines 15 and 29 as potential critical residues in the mouse PRM1 sequence, inducing the characteristic mouse “hooked” nuclear sperm shape. To explore this idea, mice with mutations in PRM1 Cys15 and Cys29 were generated. These mice remained fertile with no significant changes in sperm count or protamine expression levels. However, modifications in sperm head shape were observed. Transmission electron microscopy revealed disrupted chromatin condensation in mutant sperm, with several morphological changes and a remarkable increase in histone retention. Overall, the findings suggest that species-specific PRM1 cysteine residue positions are crucial for nuclear shape determination and histone retention in spermatozoa.

Název v anglickém jazyce

Protamine sequence determines species-specific nuclear shape and histone retention

Popis výsledku anglicky

The nuclear shape observed after the forced expression of mouse or human protamine 1 (PRM1) in fibroblasts led us to propose the hypothesis that the PRM1 sequence plays an important role in imposing the overall shape of the protaminized nucleus. Comparison of mouse and human PRM1 sequences pointed to cysteines 15 and 29 as potential critical residues in the mouse PRM1 sequence, inducing the characteristic mouse “hooked” nuclear sperm shape. To explore this idea, mice with mutations in PRM1 Cys15 and Cys29 were generated. These mice remained fertile with no significant changes in sperm count or protamine expression levels. However, modifications in sperm head shape were observed. Transmission electron microscopy revealed disrupted chromatin condensation in mutant sperm, with several morphological changes and a remarkable increase in histone retention. Overall, the findings suggest that species-specific PRM1 cysteine residue positions are crucial for nuclear shape determination and histone retention in spermatozoa.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

iScience

ISSN

2589-0042

e-ISSN

2589-0042

Svazek periodika

28

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

113102

Kód UT WoS článku

001550685300001

EID výsledku v databázi Scopus

2-s2.0-105011370909