Exprese genu pro lidský erytropoietin v mléčné žláze transgenní myši

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F01%3A00105389" target="_blank" >RIV/68378050:_____/01:00105389 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of human erythropoietin gene in the mammary gland of a transgenic mouse

Popis výsledku v původním jazyce

Whey acidic protein, WAP, is being recognized as a principal milk protein expressed in pregnant or lactating females of the several mammalian species. Recently it has been shown that 6.3kb 5Ž-untranslated region of the rabbit WAP (rWAP) gene is able to control, and almost completely restrict, the expression of transgene into the mammary gland of the transgenic animal. We cloned genomic fragment carrying the rWAP gene locus from the ?DASHII phage genomic library and used 8.5 kb long 5Ž-untranslated partof the rWAP gene to target the expression of the human erythropoietin gene (hEPO), cloned from the ?FIXII phage genomic library, into the mammary gland of the mouse. Vectors, carrying the hEPO gene or the rWAP-hEPO hybrid gene construct, were injected into the mice ova and 12 transgenic animals were identified by PCR and Southern blot from the progeny of 168 tested littermates. Transgenic mice were viable, fertile and have a normal development. Recombinant human erythropoietin was pro...

Název v anglickém jazyce

Expression of human erythropoietin gene in the mammary gland of a transgenic mouse

Popis výsledku anglicky

Whey acidic protein, WAP, is being recognized as a principal milk protein expressed in pregnant or lactating females of the several mammalian species. Recently it has been shown that 6.3kb 5Ž-untranslated region of the rabbit WAP (rWAP) gene is able to control, and almost completely restrict, the expression of transgene into the mammary gland of the transgenic animal. We cloned genomic fragment carrying the rWAP gene locus from the ?DASHII phage genomic library and used 8.5 kb long 5Ž-untranslated partof the rWAP gene to target the expression of the human erythropoietin gene (hEPO), cloned from the ?FIXII phage genomic library, into the mammary gland of the mouse. Vectors, carrying the hEPO gene or the rWAP-hEPO hybrid gene construct, were injected into the mice ova and 12 transgenic animals were identified by PCR and Southern blot from the progeny of 168 tested littermates. Transgenic mice were viable, fertile and have a normal development. Recombinant human erythropoietin was pro...

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Biologica

ISSN

0015-5500

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

187-195

Kód UT WoS článku

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EID výsledku v databázi Scopus

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