A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fce receptor.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F02%3A23033123" target="_blank" >RIV/68378050:_____/02:23033123 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fce receptor.

Popis výsledku v původním jazyce

We prepared monoclonal antibodies against lipid rafts from the rat basophilic leukemia cell line, RBL-2H3. One of the antibodies was found to recognize a novel glycosylphosphatidylinositol-anchored plasma membrane glycoprotein of 250 amino acids, designated TEC-21, containing a cysteine-rich domain homologous to those found in the urokinase plasminogen activator receptor/Ly6/snake neurotoxin family. The TEC-21 is abundant on the surface of RBL-2H3 cells (>106 molecules/cell) but is absent in numerous rat tissues except for testes. Aggregation of TEC-21 on RBL-2H3 cells induced a rapid increase in tyrosine phosphorylation of several substrates including Syk kinase and LAT adaptor, calcium flux, and release of secretory components. TEC-21-induced activation events were also observed in Fce receptorI-negative mutants of RBL-2H3 cells. The combined data suggest that TEC-21 is a novel lipid raft component of RBL-2H3 cells whose aggregation induces activation independently of Fce receptorI.

Název v anglickém jazyce

A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fce receptor.

Popis výsledku anglicky

We prepared monoclonal antibodies against lipid rafts from the rat basophilic leukemia cell line, RBL-2H3. One of the antibodies was found to recognize a novel glycosylphosphatidylinositol-anchored plasma membrane glycoprotein of 250 amino acids, designated TEC-21, containing a cysteine-rich domain homologous to those found in the urokinase plasminogen activator receptor/Ly6/snake neurotoxin family. The TEC-21 is abundant on the surface of RBL-2H3 cells (>106 molecules/cell) but is absent in numerous rat tissues except for testes. Aggregation of TEC-21 on RBL-2H3 cells induced a rapid increase in tyrosine phosphorylation of several substrates including Syk kinase and LAT adaptor, calcium flux, and release of secretory components. TEC-21-induced activation events were also observed in Fce receptorI-negative mutants of RBL-2H3 cells. The combined data suggest that TEC-21 is a novel lipid raft component of RBL-2H3 cells whose aggregation induces activation independently of Fce receptorI.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Immunology

ISSN

1460-2377

e-ISSN

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Svazek periodika

14

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

213-223

Kód UT WoS článku

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EID výsledku v databázi Scopus

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