Analysis of mouse polyomavirus mutants with lesions in the minor capsid proteins.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F02%3A23033132" target="_blank" >RIV/68378050:_____/02:23033132 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/02:11825

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of mouse polyomavirus mutants with lesions in the minor capsid proteins.

Popis výsledku v původním jazyce

Polyomavirus mutants E, Q and H, expressing non-myristylated VP2, were generated by replacing the N-terminal glycine residue with glutamic acid, glutamine or histidine, respectively. Viruses mutated in either VP2 or VP3 translation codons were also prepared. All mutated genomes, when transfected into murine host cells, gave rise to viral particles. Infectivity of VP2- and VP3- viruses was dramatically diminished, indicative of defects in the early stages of infection. No differences in number of cells expressing early or late viral antigens were observed between wild-type and E or Q myr- viruses. Furthermore, no delay in virus DNA replication was detected.The infectivity of mutants was affected not only by loss of the myristyl group on VP2, but also, and to a greater extent, by alteration of the N-terminal amino acid composition.

Název v anglickém jazyce

Analysis of mouse polyomavirus mutants with lesions in the minor capsid proteins.

Popis výsledku anglicky

Polyomavirus mutants E, Q and H, expressing non-myristylated VP2, were generated by replacing the N-terminal glycine residue with glutamic acid, glutamine or histidine, respectively. Viruses mutated in either VP2 or VP3 translation codons were also prepared. All mutated genomes, when transfected into murine host cells, gave rise to viral particles. Infectivity of VP2- and VP3- viruses was dramatically diminished, indicative of defects in the early stages of infection. No differences in number of cells expressing early or late viral antigens were observed between wild-type and E or Q myr- viruses. Furthermore, no delay in virus DNA replication was detected.The infectivity of mutants was affected not only by loss of the myristyl group on VP2, but also, and to a greater extent, by alteration of the N-terminal amino acid composition.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA204%2F00%2F0271" target="_blank" >GA204/00/0271: Ultrastrukturní studia interakcí antigenů polyomaviru se substrukturami tímto virem infikovaných buněk</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of General Virology

ISSN

0022-1317

e-ISSN

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Svazek periodika

83

Číslo periodika v rámci svazku

N/A

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

2309-2319

Kód UT WoS článku

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EID výsledku v databázi Scopus

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