Constitutive excluslion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F03%3A23033105" target="_blank" >RIV/68378050:_____/03:23033105 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Constitutive excluslion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes.

Popis výsledku v původním jazyce

Transformed phenotype of B cells infected with Theileria parva reverts on drug-induced parasite death, and the cured lymphocytes acquire a resting phenotype. Lymphocyte proliferation and activation of the transcription factor AP-1 are mediated by Src-family protein tyrosine kinases (present in membrane microdomains, GEMs) in a parasite-dependent fashion. Src-family PTKs are negatively regulated by PTK Csk complexed to tyrosine-photosphorylated transmembrane adapter protein PAG. Proliferation arrest ledto a striking increase of PAG/Cbp in GEMs increased markedly, whereas PTK Hck accumulation in GEM fractions did not alter on growth arrest. Thus, Theileria-induced lymphocyte proliferation and permanent activation of Hck may stem from down-regulation ofPAG/Cbp and the concomitant constitutive loss of the negative regulator Csk from the GEMs of transformed B cells.

Název v anglickém jazyce

Constitutive excluslion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes.

Popis výsledku anglicky

Transformed phenotype of B cells infected with Theileria parva reverts on drug-induced parasite death, and the cured lymphocytes acquire a resting phenotype. Lymphocyte proliferation and activation of the transcription factor AP-1 are mediated by Src-family protein tyrosine kinases (present in membrane microdomains, GEMs) in a parasite-dependent fashion. Src-family PTKs are negatively regulated by PTK Csk complexed to tyrosine-photosphorylated transmembrane adapter protein PAG. Proliferation arrest ledto a striking increase of PAG/Cbp in GEMs increased markedly, whereas PTK Hck accumulation in GEM fractions did not alter on growth arrest. Thus, Theileria-induced lymphocyte proliferation and permanent activation of Hck may stem from down-regulation ofPAG/Cbp and the concomitant constitutive loss of the negative regulator Csk from the GEMs of transformed B cells.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A026" target="_blank" >LN00A026: Centrum molekulární a buněčné imunologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Blood

ISSN

0006-4971

e-ISSN

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Svazek periodika

101

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1874-1881

Kód UT WoS článku

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EID výsledku v databázi Scopus

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