Enzymaticky modifikovaný lipoprotein o nízké hustotě (LDL) zvyšuje expresi CD36 v málo diferencovaných monocytech cestou závislou na receptoru gama aktivovaném peroxizomových proliferátorem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F04%3A00105319" target="_blank" >RIV/68378050:_____/04:00105319 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Enzymatically modified low-density lipoprotein upregulates CD36 in low-differentiated monocytic cells in a peroxisome proliferator-activated receptor-gamma-dependent way

Popis výsledku v původním jazyce

Peroxisome proliferator-activated receptor-g (PPARg) has been suggested to upregulate CD36. Since free oxidized polyunsaturated fatty acids are PPARg ligands, we studied the effects of LDL modified by the simultaneous action of sPLA2 and 15-lipoxygenase(15LO) on CD36 expression and PPARg activation in monocytic cells. Exposure of MM6 cells, which do not express CD36 or other scavenger receptors, to such enzymatically modified LDL (enzLDL) resulted in upregulation of CD36 surface protein and mRNA expression. Similar effects were observed with free 13-hydroperoxyoctadecadienoic acid but not its esterified counterpart. Less pronounced effects were observed with LDL modified by 15LO alone. Upregulation of CD36 was inversely correlated to the state of celldifferentiation, as showed by lower response to enzLDL of the scavenger receptor-expressing MM6-sr and THP1 cells. Importantly, LDL modified by sPLA2 and 15LO did not efficiently induce upregulation CD36 in PPARg-deficient macrophage-...

Název v anglickém jazyce

Enzymatically modified low-density lipoprotein upregulates CD36 in low-differentiated monocytic cells in a peroxisome proliferator-activated receptor-gamma-dependent way

Popis výsledku anglicky

Peroxisome proliferator-activated receptor-g (PPARg) has been suggested to upregulate CD36. Since free oxidized polyunsaturated fatty acids are PPARg ligands, we studied the effects of LDL modified by the simultaneous action of sPLA2 and 15-lipoxygenase(15LO) on CD36 expression and PPARg activation in monocytic cells. Exposure of MM6 cells, which do not express CD36 or other scavenger receptors, to such enzymatically modified LDL (enzLDL) resulted in upregulation of CD36 surface protein and mRNA expression. Similar effects were observed with free 13-hydroperoxyoctadecadienoic acid but not its esterified counterpart. Less pronounced effects were observed with LDL modified by 15LO alone. Upregulation of CD36 was inversely correlated to the state of celldifferentiation, as showed by lower response to enzLDL of the scavenger receptor-expressing MM6-sr and THP1 cells. Importantly, LDL modified by sPLA2 and 15LO did not efficiently induce upregulation CD36 in PPARg-deficient macrophage-...

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical Pharmacology

ISSN

0006-2952

e-ISSN

—

Svazek periodika

67

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

841-854

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—