Aggregated forms of bull seminal plasma proteins and their heparin-binding activity.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F04%3A23033208" target="_blank" >RIV/68378050:_____/04:23033208 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11310/04:00000371 RIV/00216208:11310/04:7032
Výsledek na webu
—
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Aggregated forms of bull seminal plasma proteins and their heparin-binding activity.
Popis výsledku v původním jazyce
Heparin-binding activity of bull seminal plasma proteins was shown to be dependent on their aggregation state. Protein fraction interacting with immobilized heparin was characterized by large polydispersity in the region of relative molecular mass of 60000 ů 100 000 while that not retained on the affinity carrier was present as aggregates with relative molecular mass >100 000. Components of heparin-binding and non-heparin-binding fractions were separated by RP HPLC and analyzed by SDS electrophoresis and N-terminal sequencing. Size exclusion chromatography of whole seminal plasma and heparin-binding proteins in the presence of D-fructose (as a component of seminal plasma) showed that the region of relative molecular masses of protein associated formswas shifted to lower values. An increase of heparin-binding activity of bull proteins, as determined by ELBA, correlates with a decrease of their aggregation state. The modulation of the aggregation state of bull proteins by seminal plasm
Název v anglickém jazyce
Aggregated forms of bull seminal plasma proteins and their heparin-binding activity.
Popis výsledku anglicky
Heparin-binding activity of bull seminal plasma proteins was shown to be dependent on their aggregation state. Protein fraction interacting with immobilized heparin was characterized by large polydispersity in the region of relative molecular mass of 60000 ů 100 000 while that not retained on the affinity carrier was present as aggregates with relative molecular mass >100 000. Components of heparin-binding and non-heparin-binding fractions were separated by RP HPLC and analyzed by SDS electrophoresis and N-terminal sequencing. Size exclusion chromatography of whole seminal plasma and heparin-binding proteins in the presence of D-fructose (as a component of seminal plasma) showed that the region of relative molecular masses of protein associated formswas shifted to lower values. An increase of heparin-binding activity of bull proteins, as determined by ELBA, correlates with a decrease of their aggregation state. The modulation of the aggregation state of bull proteins by seminal plasm
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
—
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2004
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Collection of Czechoslovak Chemical Communications
ISSN
0010-0765
e-ISSN
—
Svazek periodika
69
Číslo periodika v rámci svazku
N/A
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
15
Strana od-do
616-630
Kód UT WoS článku
—
EID výsledku v databázi Scopus
—