Charakterizace proteinů lidské semenné plasmy homolognich s kančími spermadhesiny AQN

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F05%3A00001242" target="_blank" >RIV/68378050:_____/05:00001242 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of human seminal plasma proteins homologous to boar AQN spermadhesins

Popis výsledku v původním jazyce

Spermadhesins play a role in sperm capacitation and primary contact of sperm and egg. We found human seminal plasma proteins immunobiochemically related to boar AQN and AWN spermadhesins. We further characterized the AQN spermadhesin-related proteins, designated hSA (human spermadhesin-like) proteins. We immunodetected three relative molecular-mass forms of hSA cross-reacting with rabbit antibody against AQN spermadhesins, each comprising three isoelectric isoforms. Gel chromatography of human seminal plasma indicated that hSA proteins formed high molecular-mass complexes with other hSA proteins or with lactoferrin and/or its fragments. Similarity of biochemical properties of hSA proteins and those of boar AQN spermadhesins imply their structural relatedness. However, localization of hSA on the sperm neck and tail suggests their different biological role from that of boar AQN spermadhesins located on the sperm head.

Název v anglickém jazyce

Characterization of human seminal plasma proteins homologous to boar AQN spermadhesins

Popis výsledku anglicky

Spermadhesins play a role in sperm capacitation and primary contact of sperm and egg. We found human seminal plasma proteins immunobiochemically related to boar AQN and AWN spermadhesins. We further characterized the AQN spermadhesin-related proteins, designated hSA (human spermadhesin-like) proteins. We immunodetected three relative molecular-mass forms of hSA cross-reacting with rabbit antibody against AQN spermadhesins, each comprising three isoelectric isoforms. Gel chromatography of human seminal plasma indicated that hSA proteins formed high molecular-mass complexes with other hSA proteins or with lactoferrin and/or its fragments. Similarity of biochemical properties of hSA proteins and those of boar AQN spermadhesins imply their structural relatedness. However, localization of hSA on the sperm neck and tail suggests their different biological role from that of boar AQN spermadhesins located on the sperm head.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Reproductive Immunology

ISSN

0165-0378

e-ISSN

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Svazek periodika

65

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

14

Strana od-do

33-46

Kód UT WoS článku

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EID výsledku v databázi Scopus

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