Reakce HT115, vysoce invazivní buněčné linie lidského kolorektálního adenokarcinomu, na působení butyrátu sodného a nedostatek glukózy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F05%3A00001368" target="_blank" >RIV/68378050:_____/05:00001368 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Response of HT115, a highly invasive human colorectal adenocarcinoma cell line, to sodium butyrate treatment and glucose deprivation

Popis výsledku v původním jazyce

Sodium butyrate or glucose deprivation induce a more differentiated phenotype in many cancer cells. We wanted to determine if the induction effect of butyrate and/or glucose deprivation depends on the differentiation state of individual cell lines. Sodium butyrate enhanced alkaline phosphatase activity and induced formation of an ultrastructurally more differentiated phenotype in both HT29 and HT115 cells. Interestingly, the more invasive HT115 cells responded more strongly to butyrate treatment. However, the differentiation effect of glucose deprivation was much less prominent in HT115 than in HT29 cells. Our data confirm the influence of the malignant potential of cells on their response to treatment with differentiation and apoptosis-inducing agents. Butyrate treatment also enhanced the adhesiveness of HT115 cells. Since E-cadherin was not found in these cells, while the level of CEACAM1 was increased, it means that the CEACAM1 molecules are involved in HT115 cell-cell adhesion.

Název v anglickém jazyce

Response of HT115, a highly invasive human colorectal adenocarcinoma cell line, to sodium butyrate treatment and glucose deprivation

Popis výsledku anglicky

Sodium butyrate or glucose deprivation induce a more differentiated phenotype in many cancer cells. We wanted to determine if the induction effect of butyrate and/or glucose deprivation depends on the differentiation state of individual cell lines. Sodium butyrate enhanced alkaline phosphatase activity and induced formation of an ultrastructurally more differentiated phenotype in both HT29 and HT115 cells. Interestingly, the more invasive HT115 cells responded more strongly to butyrate treatment. However, the differentiation effect of glucose deprivation was much less prominent in HT115 than in HT29 cells. Our data confirm the influence of the malignant potential of cells on their response to treatment with differentiation and apoptosis-inducing agents. Butyrate treatment also enhanced the adhesiveness of HT115 cells. Since E-cadherin was not found in these cells, while the level of CEACAM1 was increased, it means that the CEACAM1 molecules are involved in HT115 cell-cell adhesion.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/KSK5020115" target="_blank" >KSK5020115: Molekulární základy biologických transformací, komunikací a technologií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Oncology

ISSN

1019-6439

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

7

Strana od-do

793-799

Kód UT WoS článku

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EID výsledku v databázi Scopus

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