c-Myb inhibuje svalovou diferenciaci pomocí represe MyoD

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F05%3A00027483" target="_blank" >RIV/68378050:_____/05:00027483 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

c-Myb inhibits myogenic differentiation through repression of MyoD

Popis výsledku v původním jazyce

c-Myb, known to play a central role in hematopoiesis, is also an important factor involved in myogenesis. Here, we found that both c-myb and its alternatively spliced form c-mybE9A are expressed in proliferating C2C12 myoblasts and turned off in differentiating cells. Constitutive expression of exogenous c-myb in C2C12 cells inhibits their terminal differentiation. It is shown that the c-Myb protein physically interacts with MyoD, the key regulator of myogenesis, and inhibits MyoD-dependent transcription. The interaction domains are the DNA binding domain of c-Myb and the bHLH motif of MyoD. Our data suggest that in proliferating cells c-Myb binds MyoD and inhibits its transcriptional activity until cell-cell contacts are established and c-myb expression is switched off. Thus, the c-Myb protein may be one of factors ensuring that proliferating myoblasts remain undifferentiated.

Název v anglickém jazyce

c-Myb inhibits myogenic differentiation through repression of MyoD

Popis výsledku anglicky

c-Myb, known to play a central role in hematopoiesis, is also an important factor involved in myogenesis. Here, we found that both c-myb and its alternatively spliced form c-mybE9A are expressed in proliferating C2C12 myoblasts and turned off in differentiating cells. Constitutive expression of exogenous c-myb in C2C12 cells inhibits their terminal differentiation. It is shown that the c-Myb protein physically interacts with MyoD, the key regulator of myogenesis, and inhibits MyoD-dependent transcription. The interaction domains are the DNA binding domain of c-Myb and the bHLH motif of MyoD. Our data suggest that in proliferating cells c-Myb binds MyoD and inhibits its transcriptional activity until cell-cell contacts are established and c-myb expression is switched off. Thus, the c-Myb protein may be one of factors ensuring that proliferating myoblasts remain undifferentiated.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA304%2F03%2F0463" target="_blank" >GA304/03/0463: Úloha genu c-myb v časné embryogenezi obratlovců</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental Cell Research

ISSN

0014-4827

e-ISSN

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Svazek periodika

309

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

419-428

Kód UT WoS článku

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EID výsledku v databázi Scopus

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