Protein-1 sdružující se s tokoferolem zrychluje apoptózu indukovanou alfa-tokoferolsukcinátem v mesoteliomálních buňkách

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F06%3A00051236" target="_blank" >RIV/68378050:_____/06:00051236 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Tocopherol-associated protein-1 accelerates apoptosis induced by alpha-tocopheryl succinate in mesothelioma cells

Popis výsledku v původním jazyce

We studied the role of the ubiquitous tocopherol-associated protein-1 (TAP1) in apoptosis induction by alpha-tocopheryl succinate (alpha-TOS), a redox-silent analogue of vitamin E, in malignant mesothelioma (MM) cells. Over-expression of TAP1 in MM cellssensitised them to apoptosis by low doses of alpha-TOS which were sub-apoptotic for the parental cells. Apoptosis induced in TAP1-over-expressing cells was mitochondria and caspase-dependent, as suggested by dissipation of mitochondrial trans-membrane potential and inhibition by z VAD-fmk, respectively. Binding assays showed affinity of alpha-TOS for TAP1. Finally, TAP1 over-expressing cells accumulated alpha-TOS at higher levels compared to their normal counterparts. We suggest that TAP1 may act as anintracellular shuttle for alpha-TOS, promoting apoptosis initiated by this vitamin E analogue, as shown here for MM cells.

Název v anglickém jazyce

Tocopherol-associated protein-1 accelerates apoptosis induced by alpha-tocopheryl succinate in mesothelioma cells

Popis výsledku anglicky

We studied the role of the ubiquitous tocopherol-associated protein-1 (TAP1) in apoptosis induction by alpha-tocopheryl succinate (alpha-TOS), a redox-silent analogue of vitamin E, in malignant mesothelioma (MM) cells. Over-expression of TAP1 in MM cellssensitised them to apoptosis by low doses of alpha-TOS which were sub-apoptotic for the parental cells. Apoptosis induced in TAP1-over-expressing cells was mitochondria and caspase-dependent, as suggested by dissipation of mitochondrial trans-membrane potential and inhibition by z VAD-fmk, respectively. Binding assays showed affinity of alpha-TOS for TAP1. Finally, TAP1 over-expressing cells accumulated alpha-TOS at higher levels compared to their normal counterparts. We suggest that TAP1 may act as anintracellular shuttle for alpha-TOS, promoting apoptosis initiated by this vitamin E analogue, as shown here for MM cells.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical and Biophysical Research Communications

ISSN

0006-291X

e-ISSN

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Svazek periodika

343

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

1113-1117

Kód UT WoS článku

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EID výsledku v databázi Scopus

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