Dynamický gradient přenosu signálu Wnt reguluje iniciaci neurogeneze v savčím kortexu a buněčnou specifikaci v hipokampu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F07%3A00094481" target="_blank" >RIV/68378050:_____/07:00094481 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A dynamic gradient of Wnt signaling controls initiation of neurogenesis in the mammalian cortex and cellular specification in the hippocampus

Popis výsledku v původním jazyce

Neurogenesis in developing neocortex is strictly regulated. We report gradual retreat of canonical Wnt signaling in the cortex from lateral-to-medial and anterior-to-posterior as a neurogenesis prerequisite. Ectopic expression of a beta-catenin/LEF1 fusion protein maintains active canonical Wnt signaling in developing cortex and delays expression onset of neurogenic factors Pax6, Ngn2, Tbr2, and subsequent neurogenesis. Contrary to this, conditional ablation of beta-catenin accelerates expression of thesame neurogenic genes. We also show sustained canonical Wnt activity in the lateral cortex to give rise to cells with hippocampal characteristics in the cortical plate at the expense of cortical fate and to cells with dentate gyrus characteristics in hippocampus. The canonical Wnt signaling dose seems to determine cellular fate in developing cortex and hippocampus and recession of Wnt signaling seems to act as a morphogenetic gradient regulating neurogenesis in the cortex.

Název v anglickém jazyce

A dynamic gradient of Wnt signaling controls initiation of neurogenesis in the mammalian cortex and cellular specification in the hippocampus

Popis výsledku anglicky

Neurogenesis in developing neocortex is strictly regulated. We report gradual retreat of canonical Wnt signaling in the cortex from lateral-to-medial and anterior-to-posterior as a neurogenesis prerequisite. Ectopic expression of a beta-catenin/LEF1 fusion protein maintains active canonical Wnt signaling in developing cortex and delays expression onset of neurogenic factors Pax6, Ngn2, Tbr2, and subsequent neurogenesis. Contrary to this, conditional ablation of beta-catenin accelerates expression of thesame neurogenic genes. We also show sustained canonical Wnt activity in the lateral cortex to give rise to cells with hippocampal characteristics in the cortical plate at the expense of cortical fate and to cells with dentate gyrus characteristics in hippocampus. The canonical Wnt signaling dose seems to determine cellular fate in developing cortex and hippocampus and recession of Wnt signaling seems to act as a morphogenetic gradient regulating neurogenesis in the cortex.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/1M0520" target="_blank" >1M0520: Centrum aplikované genomiky</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Developmental Biology

ISSN

0012-1606

e-ISSN

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Svazek periodika

311

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

223-237

Kód UT WoS článku

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EID výsledku v databázi Scopus

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