Kultivace primárních epiteliálních buněk adenomu pacientů s dědičnou adenomální polypózou

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F08%3A00315266" target="_blank" >RIV/68378050:_____/08:00315266 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Culture of primary epithelial adenoma cells from familial adenomatous polyposis patients

Popis výsledku v původním jazyce

Colorectal tumors arise from unregulated cell proliferation of the intestinal epithelium through a multistep process, with premalignant adenomas as first step. Familial adenomatous polyposis patients carry a germline mutation in the APC gene leading to development of thousands of polyps, which when untreated lead to cancer. This study aimed to establish conditions for culture of epithelial cells composing an adenomatic structure. All colorectal specimens were obtained from FAP patients within 1-2 hrs ofsurgery. Cells were cultured by standard proceduresand characterized by immunostaining with pancytokeratin, vimentin and desmoplakin antibodies. Successful subculturing of the cell sheets took place only when dispase prepared in Ca2+ and Mg2+ free medium, was used to digest polyp tissue taken from FAP patients. By using immunostaining these cells were characterized as epithelial. The protocol we developed here provides a means of preparing cell cultures from human colorectal adenomas.

Název v anglickém jazyce

Culture of primary epithelial adenoma cells from familial adenomatous polyposis patients

Popis výsledku anglicky

Colorectal tumors arise from unregulated cell proliferation of the intestinal epithelium through a multistep process, with premalignant adenomas as first step. Familial adenomatous polyposis patients carry a germline mutation in the APC gene leading to development of thousands of polyps, which when untreated lead to cancer. This study aimed to establish conditions for culture of epithelial cells composing an adenomatic structure. All colorectal specimens were obtained from FAP patients within 1-2 hrs ofsurgery. Cells were cultured by standard proceduresand characterized by immunostaining with pancytokeratin, vimentin and desmoplakin antibodies. Successful subculturing of the cell sheets took place only when dispase prepared in Ca2+ and Mg2+ free medium, was used to digest polyp tissue taken from FAP patients. By using immunostaining these cells were characterized as epithelial. The protocol we developed here provides a means of preparing cell cultures from human colorectal adenomas.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Anticancer Research

ISSN

0250-7005

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

2A

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

000255254600034

EID výsledku v databázi Scopus

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