GAR22: A novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F09%3A00333660" target="_blank" >RIV/68378050:_____/09:00333660 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

GAR22: A novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells

Popis výsledku v původním jazyce

Thyroid hormone receptors (TRs) are ligand-dependent transcription factors with a major impact on erythroid cell development. Here we investigated TR activity on red cell gene expression using a genome-wide approach with DNA microarrays and identified TRtarget genes. Ligand-activated TR effectively accelerated red cell progenitor differentiation in vitro concomitantly with inducing growth arrest. We demonstrate that activated TR induced specific gene expression patterns of up- or down-regulated genes,including distinct clusters associated with accelerated differentiation in response to treatment. Mining for T3 induced genes identified BTEB1 and GAR22 as TR target genes. BTEB1 is a known TR target gene while GAR22, initially identified as a putative tumor suppressor, represents a novel TR target gene. We demonstrate that ectopic GAR22 expression in red cell progenitors lengthens the cell cycle and causes growth inhibition, but leaves red cell gene expression unaffected.

Název v anglickém jazyce

GAR22: A novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells

Popis výsledku anglicky

Thyroid hormone receptors (TRs) are ligand-dependent transcription factors with a major impact on erythroid cell development. Here we investigated TR activity on red cell gene expression using a genome-wide approach with DNA microarrays and identified TRtarget genes. Ligand-activated TR effectively accelerated red cell progenitor differentiation in vitro concomitantly with inducing growth arrest. We demonstrate that activated TR induced specific gene expression patterns of up- or down-regulated genes,including distinct clusters associated with accelerated differentiation in response to treatment. Mining for T3 induced genes identified BTEB1 and GAR22 as TR target genes. BTEB1 is a known TR target gene while GAR22, initially identified as a putative tumor suppressor, represents a novel TR target gene. We demonstrate that ectopic GAR22 expression in red cell progenitors lengthens the cell cycle and causes growth inhibition, but leaves red cell gene expression unaffected.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06077" target="_blank" >LC06077: Centrum chemické genetiky</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental Hematology

ISSN

0301-472X

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000265626900002

EID výsledku v databázi Scopus

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