Dazap2 modulates transcription driven by the Wnt effector TCF-4

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F09%3A00333973" target="_blank" >RIV/68378050:_____/09:00333973 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dazap2 modulates transcription driven by the Wnt effector TCF-4

Popis výsledku v původním jazyce

A typical feature of the transcriptional response induced by Wnt signaling is the involvement of Tcf/Lef factors that function in the nucleus as the principal mediators of signaling. Vertebrate Tcf/Lef proteins perform two well-characterized functions: In association with beta-catenin they activate gene expression, and in the absence of Wnt ligands they act as transcriptional repressors. In this report we reveal that the evolutionary conserved Dazap2 protein functions as a TCF-4 interacting partner. Wedemonstrate that a short region proximal to the TCF-4 HMG box mediates the interaction and that all Tcf/Lef family members associate with Dazap2. Interestingly, knockdown of Dazap2 not only reduced the activity of Wnt signaling as measured by Tcf/beta-catenin reporters but additionally altered the expression of Wnt signaling target genes. Finally, chromatin immunoprecipitation studies indicate that Dazap2 modulates the affinity of TCF-4 for its DNA recognition motif.

Název v anglickém jazyce

Dazap2 modulates transcription driven by the Wnt effector TCF-4

Popis výsledku anglicky

A typical feature of the transcriptional response induced by Wnt signaling is the involvement of Tcf/Lef factors that function in the nucleus as the principal mediators of signaling. Vertebrate Tcf/Lef proteins perform two well-characterized functions: In association with beta-catenin they activate gene expression, and in the absence of Wnt ligands they act as transcriptional repressors. In this report we reveal that the evolutionary conserved Dazap2 protein functions as a TCF-4 interacting partner. Wedemonstrate that a short region proximal to the TCF-4 HMG box mediates the interaction and that all Tcf/Lef family members associate with Dazap2. Interestingly, knockdown of Dazap2 not only reduced the activity of Wnt signaling as measured by Tcf/beta-catenin reporters but additionally altered the expression of Wnt signaling target genes. Finally, chromatin immunoprecipitation studies indicate that Dazap2 modulates the affinity of TCF-4 for its DNA recognition motif.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

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Kód UT WoS článku

000266354600021

EID výsledku v databázi Scopus

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