Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F10%3A00346752" target="_blank" >RIV/68378050:_____/10:00346752 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas

Popis výsledku v původním jazyce

Retroviruses and retrovirus-derived vectors integrate non-randomly into the genomes of host cells with specific preferences for transcribed genes, gene-rich regions, and CpG islands. To understand the genomic features that influence transcriptinal activity of integrated retroviruses, we cloned and analyzed the integration sites of avian sarcoma virus from chicken tumors. Growing progressively in dependence on high and stable expression of the transduced v-src oncogene, these tumors represent clonal expansions of cells bearing transcriptionally active replication-defective proviruses. Our analysis showed striking accumulation within or close to transcription units, particularly in genes broadly expressed in multiple tissues but not in tissue-specifically expressed genes. We infer that proviruses integrated in these genomic areas efficiently avoid transcriptional silencing and keep active for a long time during the growth of tumors.

Název v anglickém jazyce

Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas

Popis výsledku anglicky

Retroviruses and retrovirus-derived vectors integrate non-randomly into the genomes of host cells with specific preferences for transcribed genes, gene-rich regions, and CpG islands. To understand the genomic features that influence transcriptinal activity of integrated retroviruses, we cloned and analyzed the integration sites of avian sarcoma virus from chicken tumors. Growing progressively in dependence on high and stable expression of the transduced v-src oncogene, these tumors represent clonal expansions of cells bearing transcriptionally active replication-defective proviruses. Our analysis showed striking accumulation within or close to transcription units, particularly in genes broadly expressed in multiple tissues but not in tissue-specifically expressed genes. We infer that proviruses integrated in these genomic areas efficiently avoid transcriptional silencing and keep active for a long time during the growth of tumors.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Virology

ISSN

0022-538X

e-ISSN

—

Svazek periodika

84

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000276358000009

EID výsledku v databázi Scopus

—