The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F10%3A00353421" target="_blank" >RIV/68378050:_____/10:00353421 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho

Popis výsledku v původním jazyce

The transmembrane adaptor protein NTAL participates in signalosome assembly in hematopoietic cells, but its exact role in cell physiology remains enigmatic. This study shows that mast cells from NTAL-deficient mice, responding to Ag alone or in combination with SCF, exhibit reduced spreading on fibronectin, enhanced filamentous actin depolymerization and enhanced migration towards Ag relative to WT cells. No such differences between WT and NTAL(-/-) cells were observed when SCF alone was used as activator. Next the activities of two small GTPases, Rac and Rho, which are important regulators of actin polymerization were examined. Stimulation with Ag and/or SCF enhanced activity of Rac (1,2,3) in both NTAL(-/-) and WT cells. In contrast, RhoA activity decreased and this trend was much faster and more extensive in NTAL(-/-) cells, indicating a positive regulatory role of NTAL in the recovery of RhoA activity.

Název v anglickém jazyce

The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho

Popis výsledku anglicky

The transmembrane adaptor protein NTAL participates in signalosome assembly in hematopoietic cells, but its exact role in cell physiology remains enigmatic. This study shows that mast cells from NTAL-deficient mice, responding to Ag alone or in combination with SCF, exhibit reduced spreading on fibronectin, enhanced filamentous actin depolymerization and enhanced migration towards Ag relative to WT cells. No such differences between WT and NTAL(-/-) cells were observed when SCF alone was used as activator. Next the activities of two small GTPases, Rac and Rho, which are important regulators of actin polymerization were examined. Stimulation with Ag and/or SCF enhanced activity of Rac (1,2,3) in both NTAL(-/-) and WT cells. In contrast, RhoA activity decreased and this trend was much faster and more extensive in NTAL(-/-) cells, indicating a positive regulatory role of NTAL in the recovery of RhoA activity.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Immunology

ISSN

0014-2980

e-ISSN

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Svazek periodika

40

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

000284059000028

EID výsledku v databázi Scopus

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