PRR7 Is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F11%3A00365069" target="_blank" >RIV/68378050:_____/11:00365069 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1074/jbc.M110.175117" target="_blank" >http://dx.doi.org/10.1074/jbc.M110.175117</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M110.175117" target="_blank" >10.1074/jbc.M110.175117</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
PRR7 Is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis
Popis výsledku v původním jazyce
A highly conserved ransmembrane adaptor protein (TRAP) PRR7 was found to be readily up-regulated in activated human peripheral blood lymphocytes. Transient overexpression of PRR7 in Jurkat T cell line led to gradual apoptotic death dependent on the WW domain binding motif surrounding Tyr-166 in the intracellular part of PRR7. Jurkat clones with inducible expression of PRR7 (J-iPRR7) were generated. In these cells acute induction of PRR7 expression had a dual effect. It resulted in up-regulation of the transcription factor c-Jun and the activation marker CD69 as well as enhanced production of IL-2 after phorbol 12-myristate 13-acetate (PMA) and ionomycin treatment. Expression of PRR7 inhibited general tyrosine phosphorylation and calcium influx after Tcell receptor cross-linking by antibodies. PRR7 was constitutively tyrosine-phosphorylated and associated with Src. PRR7 is a potential regulator of signaling and apoptosis in activated T cells.
Název v anglickém jazyce
PRR7 Is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis
Popis výsledku anglicky
A highly conserved ransmembrane adaptor protein (TRAP) PRR7 was found to be readily up-regulated in activated human peripheral blood lymphocytes. Transient overexpression of PRR7 in Jurkat T cell line led to gradual apoptotic death dependent on the WW domain binding motif surrounding Tyr-166 in the intracellular part of PRR7. Jurkat clones with inducible expression of PRR7 (J-iPRR7) were generated. In these cells acute induction of PRR7 expression had a dual effect. It resulted in up-regulation of the transcription factor c-Jun and the activation marker CD69 as well as enhanced production of IL-2 after phorbol 12-myristate 13-acetate (PMA) and ionomycin treatment. Expression of PRR7 inhibited general tyrosine phosphorylation and calcium influx after Tcell receptor cross-linking by antibodies. PRR7 was constitutively tyrosine-phosphorylated and associated with Src. PRR7 is a potential regulator of signaling and apoptosis in activated T cells.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/1M0506" target="_blank" >1M0506: Centrum molekulární a buněčné imunologie</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Biological Chemistry
ISSN
0021-9258
e-ISSN
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Svazek periodika
286
Číslo periodika v rámci svazku
22
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
13
Strana od-do
19617-19629
Kód UT WoS článku
000291027700045
EID výsledku v databázi Scopus
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