Clonal analysis of hematopoietic progenitor cells in the zebrafish

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F11%3A00365457" target="_blank" >RIV/68378050:_____/11:00365457 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1182/blood-2011-01-331199" target="_blank" >http://dx.doi.org/10.1182/blood-2011-01-331199</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1182/blood-2011-01-331199" target="_blank" >10.1182/blood-2011-01-331199</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Clonal analysis of hematopoietic progenitor cells in the zebrafish

Popis výsledku v původním jazyce

Identification of hematopoietic progenitor cells in the zebrafish has been hindered by a lack of functional assays to gauge proliferative potential and differentiation capacity. To investigate the nature of myeloerythroid progenitor cells, we developed clonal methylcellulose assays utilizing recombinant zebrafish erythropoietin (Epo) and granulocyte colony stimulating factor (Gcsf). Epo was required to support erythroid colony formation, and Gcsf to support the formation of myeloid colonies. Colonies showed distinct morphologies and were easily scored by their expression of lineage-specific fluorescent transgenes. The majority of progenitor activity was contained within the precursor light scatter fraction, and more immature precursors were present within the lymphoid fraction. These studies provide the first report of clonal hematopoietic progenitor assays in the zebrafish, and establish the number, characteristics, and kinetics of myeloerythroid progenitors during hematopoiesis.

Název v anglickém jazyce

Clonal analysis of hematopoietic progenitor cells in the zebrafish

Popis výsledku anglicky

Identification of hematopoietic progenitor cells in the zebrafish has been hindered by a lack of functional assays to gauge proliferative potential and differentiation capacity. To investigate the nature of myeloerythroid progenitor cells, we developed clonal methylcellulose assays utilizing recombinant zebrafish erythropoietin (Epo) and granulocyte colony stimulating factor (Gcsf). Epo was required to support erythroid colony formation, and Gcsf to support the formation of myeloid colonies. Colonies showed distinct morphologies and were easily scored by their expression of lineage-specific fluorescent transgenes. The majority of progenitor activity was contained within the precursor light scatter fraction, and more immature precursors were present within the lymphoid fraction. These studies provide the first report of clonal hematopoietic progenitor assays in the zebrafish, and establish the number, characteristics, and kinetics of myeloerythroid progenitors during hematopoiesis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Blood

ISSN

0006-4971

e-ISSN

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Svazek periodika

118

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1274-1282

Kód UT WoS článku

000293510000019

EID výsledku v databázi Scopus

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