Mouse Tcf3 represses canonical Wnt signaling by either competing for beta-catenin binding or through occupation of DNA-binding sites

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00387847" target="_blank" >RIV/68378050:_____/12:00387847 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11010-012-1243-9" target="_blank" >http://dx.doi.org/10.1007/s11010-012-1243-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11010-012-1243-9" target="_blank" >10.1007/s11010-012-1243-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mouse Tcf3 represses canonical Wnt signaling by either competing for beta-catenin binding or through occupation of DNA-binding sites

Popis výsledku v původním jazyce

Tcf3 acts as a transcription factor controlling gene expression in canonical Wnt signaling. In this study we show that mouse Tcf3 represses canonical Wnt signaling in mouse neural stem cells and in human HEK 293 cells. We demonstrate that mouse Tcf3 mediates repression of both moderate and high levels of canonical Wnt signaling, by either competing with other members of the Tcf/Lef family for binding to beta-catenin, or for binding to DNA. We observed that the repressor activity of mouse Tcf3 was only relieved effectively upon simultaneous disruption of both mechanisms. Immunofluorescence of transfected HEK 293 cells showed co-localization of beta-catenin and Tcf3 in the nucleus of cells transfected with full-length Tcf3, but not in cells transfected with N-terminal deleted versions. A direct physical interaction between beta-catenin and Tcf3 in the nucleus was confirmed by co-immunoprecipitation studies. The inhibitory beta-catenin/Tcf3 interface was independent of the ability of Tcf3

Název v anglickém jazyce

Mouse Tcf3 represses canonical Wnt signaling by either competing for beta-catenin binding or through occupation of DNA-binding sites

Popis výsledku anglicky

Tcf3 acts as a transcription factor controlling gene expression in canonical Wnt signaling. In this study we show that mouse Tcf3 represses canonical Wnt signaling in mouse neural stem cells and in human HEK 293 cells. We demonstrate that mouse Tcf3 mediates repression of both moderate and high levels of canonical Wnt signaling, by either competing with other members of the Tcf/Lef family for binding to beta-catenin, or for binding to DNA. We observed that the repressor activity of mouse Tcf3 was only relieved effectively upon simultaneous disruption of both mechanisms. Immunofluorescence of transfected HEK 293 cells showed co-localization of beta-catenin and Tcf3 in the nucleus of cells transfected with full-length Tcf3, but not in cells transfected with N-terminal deleted versions. A direct physical interaction between beta-catenin and Tcf3 in the nucleus was confirmed by co-immunoprecipitation studies. The inhibitory beta-catenin/Tcf3 interface was independent of the ability of Tcf3

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Biochemistry

ISSN

0300-8177

e-ISSN

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Svazek periodika

365

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

53-63

Kód UT WoS článku

000303472400007

EID výsledku v databázi Scopus

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