Cross-talk between Tetraspanin CD9 and Transmembrane Adaptor Protein Non-T Cell Activation Linker (NTAL) in Mast Cell Activation and Chemotaxis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00392777" target="_blank" >RIV/68378050:_____/13:00392777 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1074/jbc.M112.449231" target="_blank" >http://dx.doi.org/10.1074/jbc.M112.449231</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1074/jbc.M112.449231" target="_blank" >10.1074/jbc.M112.449231</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cross-talk between Tetraspanin CD9 and Transmembrane Adaptor Protein Non-T Cell Activation Linker (NTAL) in Mast Cell Activation and Chemotaxis

Popis výsledku v původním jazyce

Chemotaxis, a process leading to movement of cells towards increasing concentrations of chemoattractants, is essential, among others, for recruitment of mast cells within target tissues where they play an important role in innate and adaptive immunity. In this study we prepared a new mAb specific for the tetraspanin CD9. Binding of the antibody to bone marrow derived mast cells (BMMCs) triggered activation events which included cell degranulation, Ca2+ response, dephosphorylation of ezrin/radixin/moesin(ERM) family proteins and potent tyrosine phosphorylation of the non-T cell activation linker (NTAL) but only weak phosphorylation of the linker for activation of T cells (LAT). As documented by electron microscopy of isolated plasma membrane sheets, CD9 colocalized with the high-affinity IgE receptor (Fc?RI) and NTAL but not with LAT. Further tests showed that both anti-CD9 antibody and its F(ab)2 fragment inhibited mast cell chemotaxis towards antigen. Experiments with BMMCs deficient

Název v anglickém jazyce

Cross-talk between Tetraspanin CD9 and Transmembrane Adaptor Protein Non-T Cell Activation Linker (NTAL) in Mast Cell Activation and Chemotaxis

Popis výsledku anglicky

Chemotaxis, a process leading to movement of cells towards increasing concentrations of chemoattractants, is essential, among others, for recruitment of mast cells within target tissues where they play an important role in innate and adaptive immunity. In this study we prepared a new mAb specific for the tetraspanin CD9. Binding of the antibody to bone marrow derived mast cells (BMMCs) triggered activation events which included cell degranulation, Ca2+ response, dephosphorylation of ezrin/radixin/moesin(ERM) family proteins and potent tyrosine phosphorylation of the non-T cell activation linker (NTAL) but only weak phosphorylation of the linker for activation of T cells (LAT). As documented by electron microscopy of isolated plasma membrane sheets, CD9 colocalized with the high-affinity IgE receptor (Fc?RI) and NTAL but not with LAT. Further tests showed that both anti-CD9 antibody and its F(ab)2 fragment inhibited mast cell chemotaxis towards antigen. Experiments with BMMCs deficient

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Svazek periodika

288

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

9801-9814

Kód UT WoS článku

000317114000021

EID výsledku v databázi Scopus

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