No evidence for cumulative effects in a Dnmt3b hypomorph across multiple generations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00422963" target="_blank" >RIV/68378050:_____/13:00422963 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00335-013-9451-5" target="_blank" >http://dx.doi.org/10.1007/s00335-013-9451-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00335-013-9451-5" target="_blank" >10.1007/s00335-013-9451-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

No evidence for cumulative effects in a Dnmt3b hypomorph across multiple generations

Popis výsledku v původním jazyce

Observations of inherited phenotypes that cannot be explained solely through genetic inheritance are increasing. Evidence points to transmission of non-DNA molecules in the gamete as mediators of the phenotypes. However, in most cases it is unclear whatthe molecules are, with DNA methylation, chromatin proteins, and small RNAs being the most prominent candidates. From a screen to generate novel mouse mutants of genes involved in epigenetic reprogramming, we produced a DNA methyltransferase 3b allele that is missing exon 13. Mice that are homozygous for the mutant allele have smaller stature and reduced viability, with particularly high levels of female post-natal death. Reduced DNA methylation was also detected at telocentric repeats and the X-linkedHprt gene. However, none of the abnormal phenotypes or DNA methylation changes worsened with multiple generations of homozygous mutant inbreeding. This suggests that in our model the abnormalities are reset each generation and the process

Název v anglickém jazyce

No evidence for cumulative effects in a Dnmt3b hypomorph across multiple generations

Popis výsledku anglicky

Observations of inherited phenotypes that cannot be explained solely through genetic inheritance are increasing. Evidence points to transmission of non-DNA molecules in the gamete as mediators of the phenotypes. However, in most cases it is unclear whatthe molecules are, with DNA methylation, chromatin proteins, and small RNAs being the most prominent candidates. From a screen to generate novel mouse mutants of genes involved in epigenetic reprogramming, we produced a DNA methyltransferase 3b allele that is missing exon 13. Mice that are homozygous for the mutant allele have smaller stature and reduced viability, with particularly high levels of female post-natal death. Reduced DNA methylation was also detected at telocentric repeats and the X-linkedHprt gene. However, none of the abnormal phenotypes or DNA methylation changes worsened with multiple generations of homozygous mutant inbreeding. This suggests that in our model the abnormalities are reset each generation and the process

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mammalian Genome

ISSN

0938-8990

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

5-6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

206-217

Kód UT WoS článku

000320659800005

EID výsledku v databázi Scopus

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