Human embryonic and induced pluripotent stem cells express TRAIL receptors and can be sensitized to TRAIL-Iiduced apoptosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00423058" target="_blank" >RIV/68378050:_____/13:00423058 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1089/scd.2013.0057" target="_blank" >http://dx.doi.org/10.1089/scd.2013.0057</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/scd.2013.0057" target="_blank" >10.1089/scd.2013.0057</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Human embryonic and induced pluripotent stem cells express TRAIL receptors and can be sensitized to TRAIL-Iiduced apoptosis

Popis výsledku v původním jazyce

Death ligands and their tumor necrosis factor receptor (TNFR) family receptors are the best-characterized and most efficient inducers of apoptotic signaling in somatic cells. In this study, we analyzed whether these prototypic activators of apoptosis arealso expressed and able to be activated in human pluripotent stem cells. We examined human embryonic stem cells (hESC) and human-induced pluripotent stem cells (hiPSC) and found that both cell types express primarily TNF-related apoptosis-inducing ligand (TRAIL) receptors and TNFR1, but very low levels of Fas/CD95. We also found that although hESC and hiPSC contain all the proteins required for efficient induction and progression of extrinsic apoptotic signaling, they are resistant to TRAIL-induced apoptosis. However, both hESC and hiPSC can be sensitized to TRAIL-induced apoptosis by co-treatment with protein synthesis inhibitors such as the anti-leukemia drug homoharringtonine (HHT). HHT treatment led to suppression of cellular FLICE

Název v anglickém jazyce

Human embryonic and induced pluripotent stem cells express TRAIL receptors and can be sensitized to TRAIL-Iiduced apoptosis

Popis výsledku anglicky

Death ligands and their tumor necrosis factor receptor (TNFR) family receptors are the best-characterized and most efficient inducers of apoptotic signaling in somatic cells. In this study, we analyzed whether these prototypic activators of apoptosis arealso expressed and able to be activated in human pluripotent stem cells. We examined human embryonic stem cells (hESC) and human-induced pluripotent stem cells (hiPSC) and found that both cell types express primarily TNF-related apoptosis-inducing ligand (TRAIL) receptors and TNFR1, but very low levels of Fas/CD95. We also found that although hESC and hiPSC contain all the proteins required for efficient induction and progression of extrinsic apoptotic signaling, they are resistant to TRAIL-induced apoptosis. However, both hESC and hiPSC can be sensitized to TRAIL-induced apoptosis by co-treatment with protein synthesis inhibitors such as the anti-leukemia drug homoharringtonine (HHT). HHT treatment led to suppression of cellular FLICE

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP301%2F10%2F1971" target="_blank" >GAP301/10/1971: Exprese, signalizace a funkce receptorů smrti v lidských embryonálních kmenových buňkách.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Stem Cells and Development

ISSN

1547-3287

e-ISSN

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Svazek periodika

22

Číslo periodika v rámci svazku

22

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

2964-2974

Kód UT WoS článku

000327010300005

EID výsledku v databázi Scopus

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